Fig. 1
From: Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica
The histones H2A, H2B, H3 and H4 are present in the basic nuclear proteins from E. histolytica. a Basic nuclear extracts from E. histolytica were incubated with anti-H3 and anti-H4 antibodies. Histones from Bos taurus were used as a positive control and incubated with the same antibodies. b Flowchart representation of the approaches used to isolate the protein present between 10 and 20 kDa using gel electrophoresis. The recovered proteins were subjected to mass spectrometry. More than 100 proteins were identified by mass spectrometry. c Four proteins showed similarity to histones H2A, H2B, H3 and H4. The rectangle indicates the peptide identified for each histone