Fig. 3
From: Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica
Nuclear localization of activation and repressive epigenetic marks in E. histolytica trophozoites. a Immunofluorescence assays with histone modification antibodies. IFAs were carried out with antibodies against specific histone modifications associated with transcriptional activation (H4 pan-acetylated, anti- H4K12ac, H4R3me1) and transcriptional repression (5-methyl-cytosine). Nuclei were stained with DAPI. The bar scale is indicated in each image. b Double IFAs using anti-mouse 5-methyl-cytosine (green) and pan-acetylated anti-rabbit H4 (red). 5-Methyl-cytosine signals were distributed in the nucleus and found to co-localize with anti-H4 pan-acetylated histone (yellow). The scale bar is given in each figure. A representative image from three independent experiments is shown