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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica

Fig. 5

Nucleolus and speckles are present in the nucleus of E. histolytica. a Western blot analysis of nuclear extracts from E. histolytica with anti-PRP6 antibody. b IFA analysis of PRP6 (green) is observed as a punctuated pattern. The nucleus was detected by DAPI (blue) staining. Anti-PRP6 antibodies give three different signals. In the first, PRP6 is located in the nuclear periphery and also in the nucleus (40 % of trophozoites). The second signal is found in the nuclear periphery (40 %), and the last signal is distributed only into the nucleus (20 %). The scale bar is indicated in each image. c IFAs were carried out with antibodies against lamin B1 (red) and PRP6 (green). The nuclei were stained with DAPI (blue). Co-localization signals (yellow) indicate that the PRP6 signal is located inside and around the nucleus (300×). d Dual color IFA with anti-mouse PRP6 (green) and anti-rabbit nucleolin (red). The PRP6 signal (green) is present around and inside the nucleus. The nucleolin signal is found at the nuclear periphery but also in one or both poles of the nucleus. The PRP6 and nucleolin signals co-localized only when PRP6 is distributed around the nucleus (yellow). e The PRP6 signal (green) is located mainly at the nuclear periphery and co-localized with nucleolin (red) when it is distributed around the nucleus or in one or both poles of the nucleus (yellow). f The PRP6 signal (green) is found inside the nucleus and is not co-localized with the nucleolin (red) signal when it is situated at the nuclear periphery in one or both poles of the nucleus

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