Table 2 Assessment of infections with Fasciola hepatica in field-collected Galba cubensis through different screening techniques. Multiplex PCR was used as the reference technique for parasite detection
Results | Screening method | ||
|---|---|---|---|
Snail dissection | FasciMol-ELISA | Multiplex PCR | |
Overall prevalence (%) (no. infected/no. examined) | 4.1 (31/755) | 5.3 (40/755) | 7.42 (56/755) |
Positive sites (ID) | 3 (V115, V121, V505) | 4 (V115, V121, V505, V108) | 4 (V115, V121, V505, V108) |
Χ 2 | Χ 2 = 13.69; P = 0.0033 | Χ 2 = 6.283; P = 0.0981 | – |
Kappa index (95 % confidence interval) | 0.6966 (0.5852–0.8080) | 0.8224 (0.7376–0.9071) | – |