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Fig. 1 | Parasites & Vectors

Fig. 1

From: Calmodulin disruption impacts growth and motility in juvenile liver fluke

Fig. 1

Expression of FhCaM1 (a), FhCaM2 (b) and FhCaM3 (c) during maintenance of juvenile Fasciola hepatica +/− serum for 7 days in vitro. CaM transcript abundance was assessed by qPCR at 1, 3, 5, and 7 days and normalised against the abundance of respective transcripts in 0 day juvenile fluke. Data represent mean ± SEM of percentage changes in target transcript abundance relative to a GAPDH reference transcript. Each bar represents data from at least five treatment replicates, 20 worms per replicate. Statistical analyses were performed using One Way ANOVA with Tukey’s post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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