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Fig. 3 | Parasites & Vectors

Fig. 3

From: Mechanisms of sex determination and transmission ratio distortion in Aedes aegypti

Fig. 3

Segregation ratio of wild-type and heterozygous offspring in crosses between heterozygous male and wild-type mosquitoes at two different hatching times. Hatched eggs were produced from mass crosses between Rock females and heterozygous males (♂M1-pro, ♂M2-pro, ♂M9-pro and ♂M10-pro, the male progenies of M1, M2, M9 and M10). a One thousand eggs from each cross, produced after the first blood meal, showed no significant deviation from the anticipated 1:1:1:1 ratio in all the four mass crosses (P > 0.05; df = 3; all X 2  ≤ 5.73). The hatching rates were in the range of 82.2–97.2 %. b Two hundred eggs produced as described in A but hatched 1 month later, showed a hatching rate of 74.5–97 % and pooled values of four mass crosses were significantly different from the anticipated 1:1:1:1 ratio due to the mortality of heterozygous females (*** P < 0.001; all X 2  ≥ 24.20; df = 3). After removing the heterozygous females from the counts, pooled values of heterozygous males, wild-type males and females in B did not deviate significantly from the anticipated 1:1:1 ratio (P > 0.05; X 2  = 0.00; df = 2). The numbers at the head of each column show the sex ratios of transgenic and wild-type mosquitoes from the same broods. The transgenic mosquitoes used for these crosses varied between 3 and 9 days old. The details of hatching rates and statistical tests are presented in Additional file 5

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