Fig. 4

Analysis of the ability of monocytes and macrophages to bind rEgAgB8 subunits. Binding assays were performed using biotinylated lipid-free rEgAgB8/1, rEgAgB8/2 and rEgAgB8/3. a Binding index of rEgAgB8 subunits (20, 50 and 100 μg/ml) to mouse peritoneal inflammatory cells is shown. Data are expressed as mean ± SEM of three independent experiments. Asterisks (*) indicate significant differences with respect to the control (OVA) according to the analysis by one-way ANOVA followed by Dunnett’s post-test (p < 0.05). Number signs (#) denotes significant differences between binding to monocytes/macrophage and binding to lymphocytes, according to one-way ANOVA analysis, followed by Tukey’s post-test (p < 0.05). (b), (c) Binding indexes of rEgAgB8 subunits to THP-1 derived monocytes and macrophages, respectively. Data are expressed as mean ± SEM of three independent experiments. Asterisks (*) indicate significant differences with respect to the control (OVA) (one-way ANOVA followed by Dunnett’s post-test, p < 0.05)