Prevalence, risk factors and multilocus genotyping of Enterocytozoon bieneusi in farmed foxes (Vulpes lagopus), Northern China

Table 1 Primers used in the study, annealing temperatures used in the PCRs and expected sizes of the PCR products

Gene	Primer	Sequence (5′-3′)	Annealing temperature (°C)	Fragment length (bp)	References
ITS	F1	GGTCATAGGGATGAAGAG	55	392	13
	R1	TTCGAGTTCTTTCGCGCTC	55
	F2	GCTCTGAATATCTATGGCT	55
	R2	ATCGCCGACGGATCCAAGTG	55
MS1	F1	CAAGTTGCAAGTTCAGTGTTTGAA	58	675	13
	R1	GATGAATATGCATCCATTGATGTT	58
	F2	TTGTAAATCGACCAAATGTGCTAT	58
	R2	GGACATAAACCACTAATTAATGTAAC	58
MS3	F1	CAAGCACTGTGGTTACTGTT	55	537	13
	R1	AAGTTAGGGCATTTAATAAAATTA	55
	F2	GTTCAAGTAATTGATACCAGTCT	55
	R2	CTCATTGAATCTAAATGTGTATAA	55
MS4	F1	GCATATCGTCTCATAGGAACA	55	885	13
	R1	GTTCATGGTTATTAATTCCAGAA	55
	F2	CGAAGTGTACTACATGTCTCT	55
	R2	GGACTTTAATAAGTTACCTATAGT	55
MS7	F1	GTTGATCGTCCAGATGGAATT	55	471	13
	R1	GACTATCAGTATTACTGATTATAT	55
	F2	CAATAGTAAAGGAAGATGGTCA	55
	R2	CGTCGCTTTGTTTCATAATCTT	55

ISSN: 1756-3305