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Table 1 In vivo RNAi screen results. Gene KDs affecting the number of developing oocysts and the prevalence of developing oocysts and/or melanised ookinetes are listed. Descriptive statistics (arithmetic mean ± standard error) and P values as results of statistical tests to compare parasite intensity and prevalence of each group with corresponding LacZ control are reported here. Upper part of the Table (above the double line): genes affecting oocyst intensity; lower part of the Table (below the double line): genes affecting prevalence of infection and/or oocyst / melanised ookinete intensities. Significant P values (P < 0.05) are reported. ns: P > 0.05

From: Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection

   

Developing oocysts

Melanised ookinetes

   

Intensity

Prevalence

Intensity

Prevalence

Gene KD

Rep.

N.

Mean ± SE

Fd

P§

%

P¶

Mean ± SE

Fd

P§

%

P¶

AGAP010658°

3

51

64.4 ± 11.8

3.1

0.0004

86

ns

0.2 ± 0.2

0.1

0.035

5

0.048

LacZ

63

20.8 ± 4.4

  

80

 

2.4 ± 1.0

  

18

 

AGAP007540

3

45

77.3 ± 12.1

2.1

0.002

84

ns

1.4 ± 0.6

0.6

ns

13

0.008

LacZ

64

37.3 ± 6.2

  

83

 

2.2 ± 1.1

  

29

 

AGAP009201

3

58

56.6 ± 9.4

1.9

0.032

81

ns

9.0 ± 5.4

6.5

0.040

25

ns

LacZ

84

30.5 ± 5.0

  

87

 

1.4 ± 0.6

  

17

 

AGAP004017

3

62

23.7 ± 5.0

0.7

0.036

69

ns

5.1 ± 1.7

0.6

ns

39

ns

LacZ

59

34.8 ± 5.5

  

76

 

8.0 ± 5.2

  

27

 

AGAP004928

3

64

32.5 ± 4.9

0.7

0.042

81

ns

3.5 ± 1.7

3.9

ns

24

ns

LacZ

68

45.9 ± 5.8

  

95

 

0.9 ± 0.3

  

19

 

AGAP004993

4

60

32.7 ± 7.9

0.6

0.002

69

0.0001

11.3 ± 4.4

1.5

ns

33

ns

LacZ

58

54.0 ± 7.5

  

85

 

7.3 ± 2.7

  

39

 

AGAP003960

3

52

18.7 ± 3.8

0.5

0.044

71

ns

4.0 ± 0.8

0.2

ns

48

ns

LacZ

47

38.5 ± 7.6

  

78

 

16.5 ± 7.1

  

39

 

AGAP011223

3

51

60.7 ± 12.9

1.4

ns

80

0.0002

9.6 ± 5.3

3.2

ns

22

0.018

LacZ

47

44.8 ± 11.5

  

96

 

3.0 ± 1.1

  

36

 

AGAP003879

3

76

40.2 ± 8.3

0.9

0.011

69

0.026

8.6 ± 2.6

1.5

0.003

40

0.0018

LacZ

71

44.3 ± 5.6

  

87

 

5.9 ± 4.3

  

19

 

AGAP012034

3

37

70.7 ± 25.9

2.0

ns

76

ns

0.7 ± 0.7

0.3

0.005

4

0.0001

LacZ

47

35.9 ± 9.5

  

81

 

2.6 ± 0.9

  

29

 
  1. Gene KD: ID of silenced genes (VectorBase Gene IDs)
  2. Rep.: number of independent replicates
  3. N.: sample size (total number of mosquitoes across replicates)
  4. Mean ± SE: arithmetic mean ± standard error (SE) of parasite intensities per midgut in each group after pooling replicate data
  5. Fd: fold difference, ratio between mean oocyst (or melanised ookinete) value of a specific gene KD and the LacZ KD
  6. P§: statistical significance according to Mann–Whitney U-Test on oocyst or melanised ookinete intensity of pools (gene-specific KD vs LacZ KD)
  7. %: prevalence (infected mosquitoes/total mosquitoes) of developing oocysts or melanised ookinetes calculated as geometric means of the prevalence of each replicate
  8. P¶: statistical significance according to Fisher’s Exact Test on oocyst or melanised ookinete prevalence (gene-specific KD vs LacZ KD)
  9. ° SNAP_ANOPHELES00000017730