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Table 1 In vivo RNAi screen results. Gene KDs affecting the number of developing oocysts and the prevalence of developing oocysts and/or melanised ookinetes are listed. Descriptive statistics (arithmetic mean ± standard error) and P values as results of statistical tests to compare parasite intensity and prevalence of each group with corresponding LacZ control are reported here. Upper part of the Table (above the double line): genes affecting oocyst intensity; lower part of the Table (below the double line): genes affecting prevalence of infection and/or oocyst / melanised ookinete intensities. Significant P values (P < 0.05) are reported. ns: P > 0.05

From: Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection

    Developing oocysts Melanised ookinetes
    Intensity Prevalence Intensity Prevalence
Gene KD Rep. N. Mean ± SE Fd P§ % P Mean ± SE Fd % P
AGAP010658° 3 51 64.4 ± 11.8 3.1 0.0004 86 ns 0.2 ± 0.2 0.1 0.035 5 0.048
LacZ 63 20.8 ± 4.4    80   2.4 ± 1.0    18  
AGAP007540 3 45 77.3 ± 12.1 2.1 0.002 84 ns 1.4 ± 0.6 0.6 ns 13 0.008
LacZ 64 37.3 ± 6.2    83   2.2 ± 1.1    29  
AGAP009201 3 58 56.6 ± 9.4 1.9 0.032 81 ns 9.0 ± 5.4 6.5 0.040 25 ns
LacZ 84 30.5 ± 5.0    87   1.4 ± 0.6    17  
AGAP004017 3 62 23.7 ± 5.0 0.7 0.036 69 ns 5.1 ± 1.7 0.6 ns 39 ns
LacZ 59 34.8 ± 5.5    76   8.0 ± 5.2    27  
AGAP004928 3 64 32.5 ± 4.9 0.7 0.042 81 ns 3.5 ± 1.7 3.9 ns 24 ns
LacZ 68 45.9 ± 5.8    95   0.9 ± 0.3    19  
AGAP004993 4 60 32.7 ± 7.9 0.6 0.002 69 0.0001 11.3 ± 4.4 1.5 ns 33 ns
LacZ 58 54.0 ± 7.5    85   7.3 ± 2.7    39  
AGAP003960 3 52 18.7 ± 3.8 0.5 0.044 71 ns 4.0 ± 0.8 0.2 ns 48 ns
LacZ 47 38.5 ± 7.6    78   16.5 ± 7.1    39  
AGAP011223 3 51 60.7 ± 12.9 1.4 ns 80 0.0002 9.6 ± 5.3 3.2 ns 22 0.018
LacZ 47 44.8 ± 11.5    96   3.0 ± 1.1    36  
AGAP003879 3 76 40.2 ± 8.3 0.9 0.011 69 0.026 8.6 ± 2.6 1.5 0.003 40 0.0018
LacZ 71 44.3 ± 5.6    87   5.9 ± 4.3    19  
AGAP012034 3 37 70.7 ± 25.9 2.0 ns 76 ns 0.7 ± 0.7 0.3 0.005 4 0.0001
LacZ 47 35.9 ± 9.5    81   2.6 ± 0.9    29  
  1. Gene KD: ID of silenced genes (VectorBase Gene IDs)
  2. Rep.: number of independent replicates
  3. N.: sample size (total number of mosquitoes across replicates)
  4. Mean ± SE: arithmetic mean ± standard error (SE) of parasite intensities per midgut in each group after pooling replicate data
  5. Fd: fold difference, ratio between mean oocyst (or melanised ookinete) value of a specific gene KD and the LacZ KD
  6. P§: statistical significance according to Mann–Whitney U-Test on oocyst or melanised ookinete intensity of pools (gene-specific KD vs LacZ KD)
  7. %: prevalence (infected mosquitoes/total mosquitoes) of developing oocysts or melanised ookinetes calculated as geometric means of the prevalence of each replicate
  8. P¶: statistical significance according to Fisher’s Exact Test on oocyst or melanised ookinete prevalence (gene-specific KD vs LacZ KD)
  9. ° SNAP_ANOPHELES00000017730