Table 1 Primer names, specificity and sequences used for the detection of Babesia DNA in fox lung exudate samples
Primer Name | Specificitya | Sequence (5’ – 3’) | Reference |
|---|---|---|---|
BT1-F | Universal Babesia – Theileria | GGTTGATCCTGCCAGTAGT | [23] |
BTH-1R | Universal Babesia – Theileria | TTGCGACCATACTCCCCCCA | |
BTH-1 F | Universal Babesia – Theileria | CCTGMGARACGGCTACCACATCT | |
BT1-R | Universal Babesia – Theileria | GCCTGCTGCCTTCCTTA | |
BTFox1F | Babesia annae | AGTTATAAGCTTTTATACAGC | Developed in the study |
BTFox1R | Babesia annae | CACTCTAGTTTTCTCAAAGTAAAATA | |
BT-Outer-R | Babesia annae | GGAAACCTTGTTACGACTTCTC | |
BT-Inner-R | Babesia annae | TTCTCCTTCCTTTAAGTGATAAG | |
600-F | Babesia annae | AGTTAAGAAGCTCGTAGTTG | |
1200-F | Babesia annae | AGGATTGACAGATTGATAGC |