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Table 2 Annealing temperatures and amplicon sizes of nested PCR reactions used for the detection of Babesia annae DNA in fox lung exudate samples

From: Detection of Babesia annae DNA in lung exudate samples from Red foxes (Vulpes vulpes) in Great Britain

Primers

Amplicon size (bp)

Annealing Temp (°C)

BT1-F and BTH-1R

1073

55

BT1-F and BT1-R

408

60

BTFox1F and BTFox1R

655

52

BT1-F and BT-Outer-R

1737

55

BT1-F and BT-Inner-R

1717

49