Fig. 2

Antigen specific IgG antibody responses to FTAg in vaccinated mice infected with L. major for 9 weeks. Groups of 7-8 mice were vaccinated twice with one of the following formulations: PBS; ALM; ALM + CpG; ALM + Pam2; ALM + Pam3 and challenged four weeks later with L. major. Plasma samples were collected after 9 weeks of infection with L. major. a Levels of IgG1 and IgG2c antibodies specific for L. major FTAg were determined by ELISA, results are displayed as Absorbance (Abs) at 450-570 nm; bars represent the mean + SEM values for the average levels of antibody (from duplicate samples for each individual) in each group. b The ratio of IgG1:IgG2c was estimated using the mean absorbance values for each individual (from supplicate values); points represent the ratio of mean antibody levels (IgG1:IgG2c) for each individual. A single experiment was performed, adequately powered to detect a difference of < 40 % between lesion sizes in different groups. Variation between groups was determined by conducting a one-way ANOVA test with Dunnet’s post hoc test to compare test groups with controls (p < 0.05 *; p < 0.005 **; p < 0.0001)