Fig. 3

The use of Pam2 adjuvant in a vaccine model for filariasis. Groups of 5 BALB/c mice were vaccinated with either a PBS control, HKBmL3 twice, 14 days apart or formulations containing BmMfE with or without the test adjuvants Pam2 or Alum once, s.c. to the nape of the neck. Fourteen days after final vaccination, mice were challenged by infection of 50 B. malayi L3 larvae by intraperitoneal injection. a & b Parasite recoveries 6 days after infection are presented, data is pooled from 2-3 individual experiments; median +/- interquartile is shown. c At the time of challenge recovery, blood and plasma were collected and plasma samples were used to measure the amount of circulating IgG antibody isotypes specific for the vaccine antigen BmMfE; levels of relative IgG1 and IgG2a are shown. d The ratio of IgG1:IgG2a levels is also shown. Data presented is pooled from 2 experiments; median averages are shown. Variation between groups was determined by conducting a Kruskall-wallis test with Dunn’s post hoc test to compare test groups with PBS controls (p < 0.05 *; p < 0.005 **; p < 0.001***)