Fig. 4

L3 specific splenocyte cytokine responses of vaccinated mice and controls in the B. malayi vaccine model. Splenocytes were recovered from vaccinated mice challenged with B. malayi L3 larvae, 6 days after challenge. Levels of cytokine (IL-4, IL-13, IFNγ and IL-5) produced by unstimulated cells were subtracted from those produced in the presence of BmL3E. Those sham vaccinated with PBS are shown (black circles), as well as those vaccinated with BmMfE, BmMfE + Pam2, and BmMfE + Alum (grey triangles, light grey inverted triangles, and dark grey diamonds respectively). Individual points represent the mean average levels of cytokine calculated from triplicate splenocyte cultures per mouse, and horizontal bars represent median averages for each group. Data presented is from one individual experiment but is representative of 2. Variation between groups was determined by conducting a Kruskall-wallis test with Dunn’s post hoc test to compare test groups with PBS controls (p < 0.05 *)