Table 2 List of primers used
Nom | Segment | Position | Sequence | Tm |
|---|---|---|---|---|
NSng | S | 31–48 | TATCATGGATTACTTTCC | 48 |
NSca | S | 841–824 | CCTTAACCTCTAATCAAC | 50 |
M1F | M | 3–22 | ACAAAGACCGGTGCAACTTC | 53.9 |
M1R | M | 1120–1140 | CCAYGCAAAGGGTATGCAAT | 53.2 |
M2F | M | 1035–1054 | TGAGGACTCTGAATTRCACCT | 48.7 |
M2R | M | 2395–2415 | TCCAGAGAGTTGAGCCTTGC | 53.3 |
MRV1a | M | 3050–3068 | CAAATGACTACCAGTCAGC | 44.6 |
MRV2g | M | 2262–2292 | GGTGGAAGGACTCTGCGA | 52.5 |
M3F | M | 2979–2998 | CAGTCCTCAGTGAGCYCATA | 46.1 |
M3R | M | 3763–3782 | TCTCGGTTCTGGRGTGTGAA | 52.5 |