Table 1 Gene and loci identity with the forward and reverse primer sequences
Gene | Gene ID | Loci ID | PCR product size(bp) | Forward primer | Reverse primer |
|---|---|---|---|---|---|
Clock | AGAP005711 | CLK-E01-267 | 119 | GTAAAATACTCTCCCGGTA | GTAAAATACTCTCCCGGTG |
Clock | AGAP005711 | CLK-E01-192 | 113 | GCTTCGTTCGAGAGAAAGGAA | GCTTCGTTCGAGAGAAAGGAG |
Clock | AGAP005711 | CLK-E01-087 | 106 | CTTGCGCACGGTCGACTTGTCCATC | CTTGCGCACGGTCGACTTGTCCATT |
Clock | AGAP005711 | CLK-E01-240 | 119 | TTCCCGATGATGAACCCGTCC | TTCCCGATGATGAACCCGTCT |
cryptochrome1 | AGAP001958 | CRY1-E04-206 | 120 | TCGACGGCGCAGCACGGA | TCGACGGCGCAGCACGGT |
cryptochrome1 | AGAP001958 | CRY1-E04-097 | 99 | CGCACGTCCATCGTTC | CGCACGTCCATCGTTT |
cryptochrome1 | AGAP001958 | CRY1-E04-240 | 120 | CTACCACCAGCAGCTGTCCA | CTACCACCAGCAGCTGTCCG |
cryptochrome1 | AGAP001958 | CRY1-E04-252 | 102 | CGACCTTGACCGACAGTTC | CGACCTTGACCGACAGTTT |
cryptochrome2 | AGAP004261 | CRY2-E05-378 | 113 | CCACTGCCATTGCCACCA | CCACTGCCATTGCCACCG |
cryptochrome2 | AGAP004261 | CRY2-E05-561 | 98 | GGCGCAGTCGCAGGAAAAC | GGCGCAGTCGCAGGAAAAT |
cryptochrome2 | AGAP004261 | CRY2-E05-501 | 100 | TGAGAATGCTGCAGCTGTGAC | TGAGAATGCTGCAGCTGTGAT |
cryptochrome2 | AGAP004261 | CRY2-E05-407 | 113 | GCCTTGTTTGGTGTCGTCAGGCA | GCCTTGTTTGGTGTCGTCAGGCG |
cryptochrome2 | AGAP004261 | CRY2-E05-045 | 85 | TCCGCTGCCGATGGTC | TCCGCTGCCGATGGTT |
cryptochrome2 | AGAP004261 | CRY2-E05-125 | 82 | CCCCAATACCGCACACCGAA | CCCCAATACCGCACACCGAG |
cryptochrome2 | AGAP004261 | CRY2-E05-351 | 102 | TATCGTGGGTCCGGGCCGCTA | TATCGTGGGTCCGGGCCGCTG |
cryptochrome2 | AGAP004261 | CRY2-E05-051 | 85 | GCGGGAAGCAATCGCA | GCGGGAAGCAATCGCG |
Cycle | AGAP005655 | CYC-E01-034 | 117 | ATTGCTGTTGGAGGGTTTA | ATTGCTGTTGGAGGGTTTG |
Cycle | AGAP005655 | CYC-E01-217 | 118 | CCACTCGTTACACCCTGAGGG | CCACTCGTTACACCCTGAGGT |
Cycle | AGAP005655 | CYC-E01-093 | 96 | GGCAGCGTCCGATTTAAGCCCA | GGCAGCGTCCGATTTAAGCCCG |
Cycle | AGAP005655 | CYC-E01-072 | 96 | GGGTAAAGTGAAGGAGCAACTC | GGGTAAAGTGAAGGAGCAACTG |
Cycle | AGAP005655 | CYC-E01-268 | 118 | ACTTTGCACTTCATCCGA | ACTTTGCACTTCATCCGG |
Cycle | AGAP005655 | CYC-E01-250 | 118 | TGGAAGAAGGAACGGCGC | TGGAAGAAGGAACGGCGA |
Cycle | AGAP005655 | CYC-E01-021 | 98 | TTGATCTTCTTGGGCAGAGC | TTGATCTTCTTGGGCAGAGT |
Pdp1 | AGAP006376 | PDP1-E02-110 | 116 | ATCGTCGCGGGACCGCTTC | ATCGTCGCGGGACCGCTTT |
Period | AGAP001856 | PER-PAS-082 | 101 | CGGCTTCCCCAAGGAC | CGGCTTCCCCAAGGAT |
Period | AGAP001856 | PER-PAS-355 | 100 | AGAAGGCGGAGATCATGAGCGGC | AGAAGGCGGAGATCATGAGCGGT |
Period | AGAP001856 | PER-PAS-202 | 114 | GGGGAAAGAGCGGCCAGAAGGAC | GGGGAAAGAGCGGCCAGAAGGAT |
Period | AGAP001856 | PER-PAS-370 | 100 | GGTGGCCGAGATGATC | GGTGGCCGAGATGATG |
Timeless | AGAP007801 | TIM-E05-075 | 111 | GCCCCGTTGACGCTGTCC | GCCCCGTTGACGCTGTCG |
Timeless | AGAP007801 | TIM-E05-087 | 111 | GTATCTGCGTTCCGATGTCG | GTATCTGCGTTCCGATGTCT |
Timeless | AGAP007801 | TIM-E05-189 | 98 | GTCCGCTACGACACAC | GTCCGCTACGACACAT |
Timeless | AGAP007801 | TIM-E05-495 | 101 | CCTACGCTGATTGCCTGGCTA | CCTACGCTGATTGCCTGGCTG |
Vrille | AGAP007801 | VRI-E02-427 | 118 | CCCCGATAAGGATGCGGCCACC | CCCCGATAAGGATGCGGCCACT |
Vrille | AGAP007801 | VRI-E02-355 | 108 | AAGTTGGCGTGCTCGTGA | AAGTTGGCGTGCTCGTGG |