Investigating associations between biting time in the malaria vector Anopheles arabiensis Patton and single nucleotide polymorphisms in circadian clock genes: support for sub-structure among An. arabiensis in the Kilombero valley of Tanzania

Table 4 STRUCTURE assignment of allele frequencies of clock gene SNPs

Locus	SNP	Cluster 1	Cluster 2	Locus	SNP	Cluster 1	Cluster 2	Locus	SNP	Cluster 1	Cluster 2
clk-e01-087	G	0.957	0.952	cyc-e01-072	C	0.099	0.094	cry2-e05-378	C	0.883	0.889
	A	0.043	0.048		G	0.901	0.906		T	0.117	0.111
clk-e01-192	C	0.865	0.890	cyc-e01-093	G	0.461	0.503	cry2-e05-407	T	0.106	0.082
	T	0.135	0.110		A	0.539	0.497		C	0.894	0.918
clk-e01-267	T	0.847	0.900	cyc-e01-034	T	0.239	0.183	cry2-e05-501	G	0.088	0.088
	C	0.153	0.100		C	0.761	0.817		A	0.912	0.912
clk-e01-240	G	0.933	0.933	cyc-e01-217	C	0.931	0.893	cry2-e05-561	C	0.975	0.971
	A	0.067	0.067		A	0.069	0.107		T	0.025	0.029
cry1-e04-097	C	0.938	0.946	cyc-e01-021	T	0.567	0.630	cry2-e05-051	T	0.913	0.911
	T	0.062	0.054		C	0.433	0.370		C	0.087	0.089
cry1-e04-206	G	0.912	0.917	cyc-e021-250	G	0.119	0.096	cry2-e05-045	C	0.421	0.407
	A	0.088	0.083		T	0.881	0.904		T	0.579	0.593
cry1-e04-240	G	0.969	0.968	cyc-e01-268	T	0.481	0.471	cry2-e05-125	G	0.859	0.888
	A	0.031	0.032	-	C	0.519	0.529		A	0.141	0.112
cry1-e04-252	G	0.771	0.764	-	-	-	-	cry2-e05-351	T	0.533	0.528
	A	0.229	0.236	-	-	-	-		C	0.467	0.472
per-pas-082	C	0.929	0.931	tim-e05-189	G	0.513	0.022	pdp1-e02-110	G	0.967	0.977
	T	0.071	0.069		A	0.487	0.978	-	A	0.033	0.023
per-pas-202	C	0.643	0.624	tim-e05-495	G	0.764	0.991	-	-	-	-
	T	0.357	0.376		A	0.236	0.009	-	-	-	-
per-pas-355	C	0.799	0.829	tim-e05-075	G	0.570	0.994	vri-e02-355	C	0.978	0.987
	T	0.201	0.171		C	0.430	0.006		T	0.022	0.013
per-pas-370	C	0.646	0.569	tim-e05-087	C	0.187	0.489	vri-e02-427	C	0.974	0.963
	G	0.354	0.431		A	0.813	0.511		T	0.026	0.037

The Timeless gene was associated with the binary clustering into clusters 1 and 2

ISSN: 1756-3305