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Table 1 List of primers and probes used in this study. Primers AL3531 and AL3535 are used in the first round and primers AL3532 and AL3534 are nested primers used in the second round to amplify part of the GP60 gene. The primers CRULib13F and CRULib13RCp in combination with probe CRULib13TMCp amplify and detect specifically a hypothetical gene of C. parvum whereas primers ChomGP60f, ChomGP60r and probe ChomGP60Tp amplify part of the GP60 and specifically detect C. hominis

From: Molecular characterization of human Cryptosporidium spp. isolates after an unusual increase in late summer 2012

Name

Sequence

5’label

3’label

ATGFOR

atgagattgtcgctcattatc

  

AL3533REV

agatatatcttggtgcg

  

AL3531

atagtctccgctgtattc

  

AL3535

ggaaggaacgatgtatct

  

AL3532

tccgctgtattctcagcc

  

AL3534

gcagaggaaccagcatc

  

CRULib13F

tccttgaaatgaatatttgtgactcg

  

CRULib13RCp

ttaatgtggtagttgcggttgaac

  

CRULib13TMCp

tatctcttcgtagcggcgta

Vic

MGB-NFQ

ChomGP60F

aaagaacaatgaagaaagccaaa

  

ChomGP60R

ggtagaaggttgggtagcactct

  

ChomGP60Tp

tcaaggtggctccaaaggagacg

Texas Red

BHQ2