Table 1 Primer information for cDNA encoding multi- epitope antigens
cDNA | Primer | Sequencea | Enzyme | |
|---|---|---|---|---|
BSjRAD23-1 | forward | 5′-CGCGAATTCATACATTCAGGCAAGG-3′ | EcoRI | |
reverse | 5′-CGCAAGCTTGGGTAGGCTAGGCT-3′ | HindIII | ||
BSj23 | forward | 5′-CGCGAATTCATGACTGGTGCTCTGGA-3′ | EcoRI | |
reverse | 5′-CGCAAGCTTCTAGTTGCGTTTTAAG-3′ | HindIII | ||
BSjPGM-BSj23 | BSjPGM | forward | 5′-CGCGGATCCTGGCGTCTAAATGAAAGA-3′ | BamHI |
reverse | 5′-CGCGAATTCAAACCAGAATGGTAGT-3′ | EcoRI | ||
BSj23 | forward | 5′-CGCGAATTCATGACTGGTGCTCTGGA-3′ | EcoRI | |
reverse | 5′-CGCAAGCTTCTAGTTGCGTTTTAAG-3′ | HindIII | ||
BSjPGM-BSjRAD23-1 | BSjPGM | forward | 5′-CGCGGATCCTGGCGTCTAAATGAAAGA-3′ | BamHI |
reverse | 5′-CGCGAATTCAAACCAGAATGGTAGT-3′ | EcoRI | ||
BSjRAD23-1 | forward | 5′-CGCGAATTCATACATTCAGGCAAGG-3′ | EcoRI | |
reverse | 5′-CGCAAGCTTGGGTAGGCTAGGCT-3′ | HindIII | ||
BSjPGM-BSjRAD23-1-BSj23 | BSj23 | forward | 5′-CGCAAGCTTATGACTGGTGCTCTGGA-3′ | HindIII |
reverse | 5′-CGCCTCGAGCTAGTTGCGTTTTAAG-3′ | XhoI | ||
BSjRAD23-2-BSjPGM-BSj23 | BSjRAD23-2 | forward | 5′-CGCGGATCCATGGTCATACGAGCAATG-3′ | BamHI |
reverse | 5′-CGCGAGCTCTGCGATTGGGTCTTCTGA-3′ | SacI | ||
BSjPGM | forward | 5′-CGGGAGCTCTGGCGTCTAAATGAAAG-3′ | SacI | |
reverse | 5′-CGCAAGCTTAAACCAGAATGGTAG-3′ | HindIII | ||
BSj23 | forward | 5′-CGCAAGCTTATGACTGGTGCTCTGGA-3′ | HindIII | |
reverse | 5′-CGCCTCGAGCTAGTTGCGTTTTAAG-3′ | XhoI | ||