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Table 1 Primer information for cDNA encoding multi- epitope antigens

From: Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay

cDNA Primer Sequencea Enzyme
BSjRAD23-1 forward 5′-CGCGAATTCATACATTCAGGCAAGG-3′ EcoRI
reverse 5′-CGCAAGCTTGGGTAGGCTAGGCT-3′ HindIII
BSj23 forward 5′-CGCGAATTCATGACTGGTGCTCTGGA-3′ EcoRI
reverse 5′-CGCAAGCTTCTAGTTGCGTTTTAAG-3′ HindIII
BSjPGM-BSj23 BSjPGM forward 5′-CGCGGATCCTGGCGTCTAAATGAAAGA-3′ BamHI
reverse 5′-CGCGAATTCAAACCAGAATGGTAGT-3′ EcoRI
BSj23 forward 5′-CGCGAATTCATGACTGGTGCTCTGGA-3′ EcoRI
reverse 5′-CGCAAGCTTCTAGTTGCGTTTTAAG-3′ HindIII
BSjPGM-BSjRAD23-1 BSjPGM forward 5′-CGCGGATCCTGGCGTCTAAATGAAAGA-3′ BamHI
reverse 5′-CGCGAATTCAAACCAGAATGGTAGT-3′ EcoRI
BSjRAD23-1 forward 5′-CGCGAATTCATACATTCAGGCAAGG-3′ EcoRI
reverse 5′-CGCAAGCTTGGGTAGGCTAGGCT-3′ HindIII
BSjPGM-BSjRAD23-1-BSj23 BSj23 forward 5′-CGCAAGCTTATGACTGGTGCTCTGGA-3′ HindIII
reverse 5′-CGCCTCGAGCTAGTTGCGTTTTAAG-3′ XhoI
BSjRAD23-2-BSjPGM-BSj23 BSjRAD23-2 forward 5′-CGCGGATCCATGGTCATACGAGCAATG-3′ BamHI
reverse 5′-CGCGAGCTCTGCGATTGGGTCTTCTGA-3′ SacI
BSjPGM forward 5′-CGGGAGCTCTGGCGTCTAAATGAAAG-3′ SacI
reverse 5′-CGCAAGCTTAAACCAGAATGGTAG-3′ HindIII
BSj23 forward 5′-CGCAAGCTTATGACTGGTGCTCTGGA-3′ HindIII
reverse 5′-CGCCTCGAGCTAGTTGCGTTTTAAG-3′ XhoI
  1. aThe sequences of the restriction enzymes are underlined