Fig. 2

Asaia ^gfp visualisation in recipient mosquito population (a) and horizontal transmission analysis in large cages in An. stephensi (b). a Optical (1, 3) and fluorescent (2, 4) images of the midgut of a female An. stephensi. Asaia ^gfp cells are clearly visible into mosquito midgut (2) from cage A after 5 days from the release of colonised males compare to mosquito from control cage C (4). Scale-bar: 200 μm. b Percentages of Asaia ^gfp positive and negative An. stephensi mosquitoes (female: F and male: M) in large cages at different days after the release of 12 and 36 paratrangenic males in cage A and B, respectively. Cage C represents the negative control. Means of three independent replicates are represented. Asterisks represent statistical significance (P < 0.001) as determined by comparisons using G-test and Bonferroni post-hoc test