Fig. 5

IL-10 and TGF-β1 mediated the inhibition of the proliferation of responder T-cells from DTH mice by SJMHE1-induced CD4⁺CD25⁺ Tregs. a Responder CD4⁺CD25⁻ T-cells (1 × 10⁵ cells/well) and irradiated APCs (1 × 10⁵ cells/well) from DTH mice (primed and challenged with OVA alone) were cultured with CD4⁺CD25⁺ T-cells (5 × 10⁴ cells/well) harvested from either SJMHE1- or PBS-treated mice and stimulated with anti-CD3 (1 μg/mL) in the presence or absence of SJMHE1 (0.1 μg/mL); b CD4⁺CD25⁻ T-cells (1 × 10⁵ cells/well) and irradiated APCs (1 × 10⁵ cells/well) from DTH mice were cultured for 72 h either alone or with CD4⁺CD25⁺ T-cells (5 × 10⁴ cells/well) from SJMHE1-immunised mice and stimulated with anti-CD3 (1 μg/mL). Wells contained either anti-IL-10 (3 μg/mL), anti-TGF-β1 (0.5 μg/mL), both antibodies, or isotype control antibodies. Proliferation was measured by ³H-thymidine incorporation for the last 16 h of the experiment. Data are shown as the mean ± SD (n = 6 per group) from three independent experiments. Asterisks indicate significant differences analysed using the independent Student’s t-test (***P < 0.001)