Fig. 6

SJMHE1 induced the generation of peripheral CD4⁺CD25⁺ Tregs from CD4⁺CD25⁻ T-cells. BALB/c mice were injected with anti-CD25 Ab to eliminate CD25⁺ T-cells and sensitised with OVA alone or combined with 30 μg of SJMHE1 after 24 h. a DTH responses were assessed over the subsequent 24 h with the change in ear thickness. DTH responses are expressed as the mean ± SD of 12 mice from two independent experiments; b Challenge with OVA occurred 7 days later; 24 h after the challenge, spleen and LNs from each mouse were pooled. FCM analysis for CD3, CD4, CD25, and Foxp3 was performed, and data are expressed as the mean ± SD of 18 mice from three independent experiments; c One representative experiment of the total data shown in b; d Splenic CD4⁺CD25⁻ T-cells (2 × 10⁵/well) isolated from DTH mice (primed and challenged with OVA alone) were stimulated with 0.1 μg/mL SJMHE1 in the absence or presence of APC (2 × 10⁵/well) for 3 days. The cells were stained with PerCP-anti-CD3, FITC-anti-CD4, APC-anti-CD25, and PE-anti-Foxp3 and then examined by flow cytometry. Data are expressed as the mean ± SD of two independent experiments (n = 6 per group); e Representative experiment of the total data shown in d. Asterisks indicate significant differences analysed using the independent Student’s t-test. (**P < 0.01; ***P < 0.001)