Fig. 2
From: Establishment of transient and stable transfection systems for Babesia ovata
Schematic diagram of GFP-expressing plasmid construct, growth inhibition of B. ovata by WR99210 and fluorescence microscopy images of stably expressing GFP B. ovata. a Plasmid construct for stable GFP expression (pBS-EGRADE) showing the recombination sites for integration into the ef-1α locus by homologous double cross-over recombination. The restriction sites for linearization (SalI and SacII) are shown. b Growth inhibition rate of B. ovata in the absence or presence of different concentrations of WR99210. All data are expressed as mean + SEM of triplicate culture. c Live fluorescence microscopy images of GFP-expressing parasites. The pictures are taken from the pBS-EGRADE genome integrated isolate 1. The parasite nucleus was stained with Hoechst33342. Scale-bar: 10 μm