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Fig. 3 | Parasites & Vectors

Fig. 3

From: Establishment of transient and stable transfection systems for Babesia ovata

Fig. 3

Confirmation of the integration of pBS-EGRADE into the ef-1α locus. a Schematic diagram and PCRs to confirm the integration of pBS-EGRADE into ef-1α locus. PCR-1, −2 and −3 are done with primer sets Boef1α-integ-F/eGFP-R-HindIII-IF, hDHFR-F-BamHI-IF/Boef1α-integ-R and Boef1α-F2/Boef1α-integ-R, respectively. b Schematic diagram and Southern blot analysis to confirm the integration of pBS-EGRADE into ef-1α locus. Five microgram of samples genomic DNA were digested with PacI and NotI, and hybridized with gfp and ef-1α 3’NR probes. GI: genome integrated; Episomal: two independent transfectants with circular plasmids; WT: wild type

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