Table 1 List of primers used to construct GFP-expressing plasmid, pBS-EGRADE
From: Establishment of transient and stable transfection systems for Babesia ovata
Primer | Sequence |
|---|---|
BoefIG2-F-ClaI-IF | AGGTCGACGGTATCGATCACTCATTTAGATTGCGA |
BoefIG2-R-ClaI-IF | ATATCAAGCTTATCGATCTTGTTTAAGGTTTAACGA |
eGFP-F-HindIII-IF | CAAGATCGATAAGCTTATGGTGAGCAAGGGCGAGGA |
eGFP-R-HindIII-IF | TCCCGATATCAAGCTTTTACTTGTACAGCTCGTCCA |
BoRAPIG-F-EcoRV-IF | ATCGATAAGCTTGATATCGGGAGCAGAGCTCTGGCCA |
BoRAPIG-R-EcoRV-IF | CTGCAGGAATTCGATATCCGTAAAGCGAGACGGGGA |
BoActin5UTR-F-SmaI-IF | GAATTCCTGCAGCCCGGGCACATAAAGGTACCTATGCT |
BoActin5UTR-R-SmaI-IF | ACTAGTGGATCCCCCGGGCTTGGCGAAATTTTACTCCT |
hDHFR-F-BamHI-IF | CAAGCCCGGGGGATCCATGGTTGGTTCGCTAAACT |
hDHFR-R-BamHI-IF | ACGTACTAGTGGATCCTTAATCATTCTTCTCATATAC |
Boef3UTR-F-SpeI-IF | CGGGGGATCCACTAGTACGTTGCGCCTTTAGGTGCA |
Boef3UTR-R-SpeI-IF | CCGCTCTAGAACTAGTGGTGTGAGCATTGCTTGTC |
BoADE-F-EcoRI-IF | TACGGATATCGAATTCCTGCAGCCCGGGCACATAAAGG |
BoADE-R-EcoRI-IF | CGGGCTGCAGGAATTCACTAGTGGTGTGAGCATTGC |