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Fig. 1 | Parasites & Vectors

Fig. 1

From: In vitro permissiveness of bovine neutrophils and monocyte derived macrophages to Leishmania donovani of Ethiopian isolate

Fig. 1

Whole blood and stationary phase promastigotes of L. donovani co-incubated for 22 h. Erythrocytes were lysed, and remaining cells were washed twice in PBS (pH 7.2). Cytospin smears from cell suspensions were stained with 10 % Giemsa staining solution, and examined for PMN infection by microscopic observation and enumeration of amastigote like forms inside them. PMN from Zebu-Holstein crossed animals are shown in panels (a-c), and from Zebu animals in panels (d-f). Panel a, d: uninfected PMN control; panels b, c, d, f: PMN infected with L. donovani parasites, designated by the red arrows, at 22 h of culture. The original photomicrographs were taken at 100× magnification

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