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Fig. 6 | Parasites & Vectors

Fig. 6

From: In vitro permissiveness of bovine neutrophils and monocyte derived macrophages to Leishmania donovani of Ethiopian isolate

Fig. 6

PCR products after ITS-1 primer amplification and HhaI digestion of promastigote and amastigote DNA. Panel a depicts primer amplification products as follows. Lane 1:100 bp Ladder; Lane 2: L. donovani reference strain; Lane 3: L. donovani promastigotes retrieved from MDM; Lane 4: L. donovani amastigote within MDM; Lane 5: L. aethiopica reference strain; Lane 6: L. aethiopica culture promastigotes; Lane 7: L. major; Lane 8: L. tropica; Lane 9: L. infantum; Lane10: NC (TE buffer). Panel b illustrates HhaI-digested ITS-1 amplicons as follows. Lane 1: 100 bp ladder; Lane 2: L. donovani reference strain; Lane 3: L. donovani cultured promastigotes; Lane 4: L. donovani cultured amastigotes; Lane 5: L. aethiopica reference strain; Lane 6: L. aethiopica culture promastigotes; Lane 7: L. major; Lane 8: L. tropica; Lane 9: L. infantum; Lane 10: NC (TE buffer)

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Parasites & Vectors

ISSN: 1756-3305

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