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Table 2 RNAi phenotypes observed in the progeny of H. bacteriophora worms injected with dsRNA

From: RNAi-mediated gene knockdown by microinjection in the model entomopathogenic nematode Heterorhabditis bacteriophora

 

Trial-1

Trial-2

Trial-3

Average

GFP

0 (0/53)

0 (0/95)

0 (0/127)

0 (0/127)

nol-5 (ste)

20 (35/173)

25 (29/113)

60 (92/152)

36 (156/438)

cct-2 (ste)

44 (74/165)

50 (91/179)

51 (102/198)

50 (267/542)

dpy-13 (dpy)

7 (3/42)

8 (2/25)

6 (3/43)

7 (8/110)

dpy-7 (dpy)

3 (1/26)

5 (3/53)

2 (2/98)

3 (6/177)

  1. For each trial, percentage of observed phenotype is followed in parenthesis by total number of progeny that had the predicted phenotype over total number of progeny from the injection. Last column is the average of all the trials. Ste (Sterile) and dpy (dumpy)
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Parasites & Vectors

ISSN: 1756-3305

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