Fig. 2
Recombinant PbPH protein purification and immunization. a Purified recombinant proteins were subjected to electrophoresis on a 12 % SDS-PAGE gel. M: PageRuler Prestained Protein Ladder in kDa. b ELISA showing the PbPH-antibody titers after immunizations. Serum samples were collected post-immunization on days 14, 28, and 38. Titers correspond to the last dilution of the test serum in which OD490 values were above that of the cut-off. The cut-off value was defined as that of pooled sera from native mice. Serum samples were tested at two-fold serial dilutions (1 × 102–2 × 105). Pooled sera from native mice were used as a negative control. Results are representative of three independent experiments. The error bars indicate mean ± SEM (n = 3). **P < 0.01 (Student’s t test)