Characterization of a Plasmodium berghei sexual stage antigen PbPH as a new candidate for malaria transmission-blocking vaccine

Table 1 Mosquito infection prevalence and oocyst intensity after feeding on control mice and mice immunized with recombinant PbPH

	Control mice ^a					PbPH-immunized mice ^a
	M1	M2	M3	M4	M5	M1	M2	M3	M4	M5
Mosquitoes Infected/Dissected	28/25	28/27	29/27	30/29	29/27	30/24	32/27	31/25	30/24	32/25
Prevalence of infection (%) ^b	89.3	96.4	93.1	96.7	93.1	80	84.4	80.6	80	78.1
Mean prevalence (%)					93.72					80.62
Reduction in prevalence (%)^c										13.1
Oocyst intensity ^d	96.5	98.70	106.80	106.60	93.97	54.10	49.53	46.65	58.39	52.94
SEM ^e	16.04	13.68	16.08	16.08	16.78	9.20	7.66	7.74	9.54	9.68
Mean oocyst intensity					100.51					52.32
Reduction in oocyst intensity ^f										47.95

^a Five mice were used in each group for the evaluation of TB activity
^b The prevalence of infection was calculated as the number of mosquitoes with oocysts/total mosquitoes dissected in each group × 100%
^c The percent reduction of prevalence was calculated as % mean prevalence _control – % mean prevalence _PbPH
^d Mean number of oocysts per mosquito midgut
^e Standard error of the mean
^f The percent reduction in oocyst intensity was calculated as (mean oocyst intensity_control – mean oocyst intensity_PbPH)/mean oocyst intensity _control × 100%

ISSN: 1756-3305