Fig. 3From: Imipramine alters the sterol profile in Leishmania amazonensis and increases its sensitivity to miconazoleAntiamastigote activity of the combination of imipramine and miconazole. Peritoneal macrophages infected with L. amazonensis that were left untreated or treated with imipramine and miconazole were incubated for 72Ā hours at 37Ā Ā°C. After incubation, the cells were fixed and stained. The infection rate was calculated using the formula: % infected MĆ x number of amastigotes/total No. MĆ. a imipramine b miconazoleā+ā12.5Ā Ī¼M imipramine. The experiments were performed in triplicate, nā=ā3. The graphs are representative of one experiment, and the standard deviations are shown. * Pā<ā0.05, ** Pā<ā0.01, *** P <Ā 0.001Back to article page