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Table 1 Patient dengue virus (DENV) viraemia, serotype and midgut/salivary gland infection rates and virus titre in Aedes aegypti exposed using either direct skin feeding assay (DSFA) on patients or membrane feeding assay using EDTA-treated patient blood (MFA)

From: Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Patient

Serotype

Serum virus titre (log10 RNAcopy/ml)

Mode of infection

Feeding rate fed/n (%)

P-valuea

Midgut

Salivary Glands

Infected/n (% infection)b

Number of RNA copies

P-valuea

infected/n (% infection)b

DENV SG Titer (Log10TCID50/ml)

P-valuea

5

1

9.49

DSFA

30/30 (100)

<0.0001*

100 (12)

9.07 ± 0.26

0.95

12/12 (100)

4.98 ± 1.03

0.16

MFA

37/56 (66)

100 (12)

9.11 ± 0.13

12/12 (100)

5.52 ± 0.66

8

1

9.21

DSFA

30/30 (100)

<0.0001*

9/9 (100)

9.16 ± 0.09

0.02*

9/9(100)

4.67 ± 0.94

0.02*

MFA

27/56 (48.2)

12/12 (100)

8.45 ± 0.54

12/12(100)

5.24 ± 0.28

9

1

5.65

DSFA

27/30 (90)

0.03*

12/12 (100)

3.62 ± 1.93

0.88

0/12

0

ND

MFA

38/56 (67.8)

8/12 (66.7)

4.57 ± 3.10

0/12

0

10

1

4.76

DSFA

30/30 (100)

0.007*

2/12 (16.7)

3.52

ND

0/12

0

ND

MFA

45/56 (80.4)

2/12 (16.7)

3.08

0/12

0

11

1

7.51

DSFA

29/30 (96.7)

0.25

12/12 (100)

6.22 ± 2.96

0.52

6/12 (50)

3.95 ± 0.7

0.09

MFA

49/56 (87.5)

12/12 (100)

6.38 ± 3.3

5/12 (42)

4.95 ± 0.76

12

1

9.18

DSFA

28/30 (93.3)

1

12/12 (100)

9.8 ± 0.48

0.52

12/12 (100)

5.18 ± 0.49

0.003

MFA

51/56 (91.1)

12/12 (100)

9.66 ± 0.31

8/12 (67)

4.74 ± 1.66

13

1

8.44

DSFA

17/17 (100)

0.57

12/12 (100)

9.69 ± 0.37

0.06

11/12 (92)

3.52 ± 1.19

0.82

MFA

43/47 (91.5)

12/12 (100)

9.73 ± 1.51

12/12 (100)

4.89 ± -.66

14

1

5.71

DSFA

17/17 (100)

0.001*

7/12 (58.3)

3.25 ± 1.12

1

0/12

0

ND

MFA

27/46 (58.7)

8/12 (66.7)

2.83 ± 0.2

0/12

0

15

1

5.64

DSFA

16/17 (94.1)

0.66

11/11 (100)

5.21 ± 3.00

0.14

3/12 (27)

4.19 ± 0.57

ND

MFA

37/42 (88.1)

9/12 (75)

3.87 ± 2.01

1/11 (9)

3.95

16

1

8.85

DSFA

17/17 (100)

1

12/12 (100)

9.50 ± 0.11

0.02*

12/12 (100)

4.64 ± 0.49

0.8

MFA

46/46 (100)

12/12 (100)

9.66 ± 0.17

12/12 (100)

4.34 ± 0.67

17

1

4.75

DSFA

17/17 (100)

0.02*

8/12 (66.7)

2.62 ± 0.01

0.16

0/12

0

ND

MFA

40/56 (71.4)

7/12 (58.3)

3.48 ± 0.82

0/12

0

18

1

7.55

DSFA

17/17 (100)

0.03*

12/12 (100)

7.6 ± 3.68

0.06

1/12 (8)

4.95

ND

MFA

42/56 (75)

12/12 (100)

5.44 ± 3.29

6/12 (50)

3.73 ± 0.30

19

1

7.31

DSFA

17/17 (100)

0.02*

10/12 (83.3)

5.9 ± 3.26

0.45

5/12 (45)

4.69 ± 0.74

0.76

MFA

39/54 (72.2)

10/11 (90.9)

6.89 ± 3.17

3/12 (25)

4.52 ± 1.00

20

1

8.2

DSFA

17/17 (100)

0.32

12/12 (100)

9.66 ± 0.24

0.15

12/12 (100)

4.25 ± 0.49

0.54

MFA

50/56 (89.3)

12/12 (100)

9.51 ± 0.18

12/12 (100)

4.36 ± 0.52

21

1

8.79

DSFA

17/17 (100)

0.19

12/12 (100)

8.86 ± 0.72

0.4529

12/12 (100)

4.22 ± 0.41

0.63

MFA

49/56 (87.5)

12/12 (100)

9.12 ± 0.22

12/12 (100)

4.26 ± 0.59

22

1

8.84

DSFA

17/17 (100)

0.02*

12/12 (100)

9.17 ± 0.12

0.05

12/12 (100)

5.02 ± 0.34

0.033

MFA

40/56 (71.4)

12/12 (100)

9.37 ± 0.27

12/12 (100)

4.52 ± 0.65

23

1

9.39

DSFA

17/17 (100)

0.58

12/12 (100)

9.05 ± 0.21

0.1489

12/12 (100)

3.58 ± 0.71

0.42

MFA

51/56 (91.1)

12/12 (100)

9.14 ± 0.41

12/12 (100)

3.9 ± 0.44

24

1

6.31

DSFA

17/17 (100)

0.17

12/12 (100)

5.58 ± 2.83

0.8399

3/12 (17)

3.84 ± 1.33

ND

MFA

42/56 (75)

12/12 (100)

6.1 ± 2.85

6/12 (50)

4.40 ± 0.39

25

1

8.12

DSFA

17/17 (100)

1

12/12 (100)

9.26 ± 0.46

0.5637

12/12 (100)

4.75 ± 0.58

0.29

MFA

55/58 (94.8)

12/12 (100)

9.34 ± 0.26

12/12 (100)

4.48 ± 0.46

26

1

8.03

DSFA

17/17 (100)

1

12/12 (100)

9.03 ± 0.34

0.3865

12/12 (100)

4.99 ± 0.50

0.4

MFA

55/56 (98.2)

12/12 (100)

8.16 ± 2.26

10/12 (83)

5.15 ± 0.41

4

2

5.6

DSFA

29/30 (96.7)

1

7/12 (58.3)

3.46 ± 2.34

1

1/12(8.3)

4.52

ND

MFA

55/56 (98)

7/12 (58.3)

3.52 ± 2.12

1/12(8.3)

2.52

7

2

3.73

DSFA

29/29 (100)

0.09

11/12 (91.7)

3.04 ± 0.52

0.21

0/12

0

ND

MFA

51/58 (87.9)

9/12 (75)

3.42 ± 0.65

0/12

0

1

3

3.69

DSFA

30/30 (100)

0.15

1/12 (8.3)

2.47

ND

0/12

0

ND

MFA

47/56 (83.9)

2/12 (16.7)

3.11

0/12

0

2

3

3.94

DSFA

30/30 (100)

<0.0001*

3/12 (25)

1.68 ± 0.38

ND

0/12

0

ND

MFA

39/59 (69.6)

2/12 (16.7)

1.41

0/12

0

3

3

8.69

DSFA

30/30 (100)

0.0001*

12/12 (100)

9.23 ± 0.18

0.15

12/12 (100)

5.22 ± 1.26

0.16

MFA

40/60 (66.7)

12/12 (100)

9.34 ± 0.18

12/12 (100)

4.54 ± 1.14

  1. aFisher's Exact Test (P- value < 0.05 are highlighted*), bPairwise comparison of midgut and salivary glands infection rate from each patient showed no significant differences by Fisher's Exact Test, ND Not done