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Table 1 Patient dengue virus (DENV) viraemia, serotype and midgut/salivary gland infection rates and virus titre in Aedes aegypti exposed using either direct skin feeding assay (DSFA) on patients or membrane feeding assay using EDTA-treated patient blood (MFA)

From: Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Patient Serotype Serum virus titre (log10 RNAcopy/ml) Mode of infection Feeding rate fed/n (%) P-valuea Midgut Salivary Glands
Infected/n (% infection)b Number of RNA copies P-valuea infected/n (% infection)b DENV SG Titer (Log10TCID50/ml) P-valuea
5 1 9.49 DSFA 30/30 (100) <0.0001* 100 (12) 9.07 ± 0.26 0.95 12/12 (100) 4.98 ± 1.03 0.16
MFA 37/56 (66) 100 (12) 9.11 ± 0.13 12/12 (100) 5.52 ± 0.66
8 1 9.21 DSFA 30/30 (100) <0.0001* 9/9 (100) 9.16 ± 0.09 0.02* 9/9(100) 4.67 ± 0.94 0.02*
MFA 27/56 (48.2) 12/12 (100) 8.45 ± 0.54 12/12(100) 5.24 ± 0.28
9 1 5.65 DSFA 27/30 (90) 0.03* 12/12 (100) 3.62 ± 1.93 0.88 0/12 0 ND
MFA 38/56 (67.8) 8/12 (66.7) 4.57 ± 3.10 0/12 0
10 1 4.76 DSFA 30/30 (100) 0.007* 2/12 (16.7) 3.52 ND 0/12 0 ND
MFA 45/56 (80.4) 2/12 (16.7) 3.08 0/12 0
11 1 7.51 DSFA 29/30 (96.7) 0.25 12/12 (100) 6.22 ± 2.96 0.52 6/12 (50) 3.95 ± 0.7 0.09
MFA 49/56 (87.5) 12/12 (100) 6.38 ± 3.3 5/12 (42) 4.95 ± 0.76
12 1 9.18 DSFA 28/30 (93.3) 1 12/12 (100) 9.8 ± 0.48 0.52 12/12 (100) 5.18 ± 0.49 0.003
MFA 51/56 (91.1) 12/12 (100) 9.66 ± 0.31 8/12 (67) 4.74 ± 1.66
13 1 8.44 DSFA 17/17 (100) 0.57 12/12 (100) 9.69 ± 0.37 0.06 11/12 (92) 3.52 ± 1.19 0.82
MFA 43/47 (91.5) 12/12 (100) 9.73 ± 1.51 12/12 (100) 4.89 ± -.66
14 1 5.71 DSFA 17/17 (100) 0.001* 7/12 (58.3) 3.25 ± 1.12 1 0/12 0 ND
MFA 27/46 (58.7) 8/12 (66.7) 2.83 ± 0.2 0/12 0
15 1 5.64 DSFA 16/17 (94.1) 0.66 11/11 (100) 5.21 ± 3.00 0.14 3/12 (27) 4.19 ± 0.57 ND
MFA 37/42 (88.1) 9/12 (75) 3.87 ± 2.01 1/11 (9) 3.95
16 1 8.85 DSFA 17/17 (100) 1 12/12 (100) 9.50 ± 0.11 0.02* 12/12 (100) 4.64 ± 0.49 0.8
MFA 46/46 (100) 12/12 (100) 9.66 ± 0.17 12/12 (100) 4.34 ± 0.67
17 1 4.75 DSFA 17/17 (100) 0.02* 8/12 (66.7) 2.62 ± 0.01 0.16 0/12 0 ND
MFA 40/56 (71.4) 7/12 (58.3) 3.48 ± 0.82 0/12 0
18 1 7.55 DSFA 17/17 (100) 0.03* 12/12 (100) 7.6 ± 3.68 0.06 1/12 (8) 4.95 ND
MFA 42/56 (75) 12/12 (100) 5.44 ± 3.29 6/12 (50) 3.73 ± 0.30
19 1 7.31 DSFA 17/17 (100) 0.02* 10/12 (83.3) 5.9 ± 3.26 0.45 5/12 (45) 4.69 ± 0.74 0.76
MFA 39/54 (72.2) 10/11 (90.9) 6.89 ± 3.17 3/12 (25) 4.52 ± 1.00
20 1 8.2 DSFA 17/17 (100) 0.32 12/12 (100) 9.66 ± 0.24 0.15 12/12 (100) 4.25 ± 0.49 0.54
MFA 50/56 (89.3) 12/12 (100) 9.51 ± 0.18 12/12 (100) 4.36 ± 0.52
21 1 8.79 DSFA 17/17 (100) 0.19 12/12 (100) 8.86 ± 0.72 0.4529 12/12 (100) 4.22 ± 0.41 0.63
MFA 49/56 (87.5) 12/12 (100) 9.12 ± 0.22 12/12 (100) 4.26 ± 0.59
22 1 8.84 DSFA 17/17 (100) 0.02* 12/12 (100) 9.17 ± 0.12 0.05 12/12 (100) 5.02 ± 0.34 0.033
MFA 40/56 (71.4) 12/12 (100) 9.37 ± 0.27 12/12 (100) 4.52 ± 0.65
23 1 9.39 DSFA 17/17 (100) 0.58 12/12 (100) 9.05 ± 0.21 0.1489 12/12 (100) 3.58 ± 0.71 0.42
MFA 51/56 (91.1) 12/12 (100) 9.14 ± 0.41 12/12 (100) 3.9 ± 0.44
24 1 6.31 DSFA 17/17 (100) 0.17 12/12 (100) 5.58 ± 2.83 0.8399 3/12 (17) 3.84 ± 1.33 ND
MFA 42/56 (75) 12/12 (100) 6.1 ± 2.85 6/12 (50) 4.40 ± 0.39
25 1 8.12 DSFA 17/17 (100) 1 12/12 (100) 9.26 ± 0.46 0.5637 12/12 (100) 4.75 ± 0.58 0.29
MFA 55/58 (94.8) 12/12 (100) 9.34 ± 0.26 12/12 (100) 4.48 ± 0.46
26 1 8.03 DSFA 17/17 (100) 1 12/12 (100) 9.03 ± 0.34 0.3865 12/12 (100) 4.99 ± 0.50 0.4
MFA 55/56 (98.2) 12/12 (100) 8.16 ± 2.26 10/12 (83) 5.15 ± 0.41
4 2 5.6 DSFA 29/30 (96.7) 1 7/12 (58.3) 3.46 ± 2.34 1 1/12(8.3) 4.52 ND
MFA 55/56 (98) 7/12 (58.3) 3.52 ± 2.12 1/12(8.3) 2.52
7 2 3.73 DSFA 29/29 (100) 0.09 11/12 (91.7) 3.04 ± 0.52 0.21 0/12 0 ND
MFA 51/58 (87.9) 9/12 (75) 3.42 ± 0.65 0/12 0
1 3 3.69 DSFA 30/30 (100) 0.15 1/12 (8.3) 2.47 ND 0/12 0 ND
MFA 47/56 (83.9) 2/12 (16.7) 3.11 0/12 0
2 3 3.94 DSFA 30/30 (100) <0.0001* 3/12 (25) 1.68 ± 0.38 ND 0/12 0 ND
MFA 39/59 (69.6) 2/12 (16.7) 1.41 0/12 0
3 3 8.69 DSFA 30/30 (100) 0.0001* 12/12 (100) 9.23 ± 0.18 0.15 12/12 (100) 5.22 ± 1.26 0.16
MFA 40/60 (66.7) 12/12 (100) 9.34 ± 0.18 12/12 (100) 4.54 ± 1.14
  1. aFisher's Exact Test (P- value < 0.05 are highlighted*), bPairwise comparison of midgut and salivary glands infection rate from each patient showed no significant differences by Fisher's Exact Test, ND Not done