Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Tan, Cheong-Huat; Wong, Pei-Sze Jeslyn; Li, Mei-Zhi Irene; Yang, Hui-Ting; Chong, Chee-Seng; Lee, Linda K.; Yuan, Shi; Leo, Yee-Sin; Ng, Lee-Ching; Lye, David C.

doi:10.1186/s13071-016-1469-6

Table 1 Patient dengue virus (DENV) viraemia, serotype and midgut/salivary gland infection rates and virus titre in Aedes aegypti exposed using either direct skin feeding assay (DSFA) on patients or membrane feeding assay using EDTA-treated patient blood (MFA)

From: Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Patient	Serotype	Serum virus titre (log₁₀ RNAcopy/ml)	Mode of infection	Feeding rate fed/n (%)	P-value^a	Midgut			Salivary Glands
Patient	Serotype	Serum virus titre (log₁₀ RNAcopy/ml)	Mode of infection	Feeding rate fed/n (%)	P-value^a	Infected/n (% infection)^b	Number of RNA copies	P-value^a	infected/n (% infection)^b	DENV SG Titer (Log₁₀TCID₅₀/ml)	P-value^a
5	1	9.49	DSFA	30/30 (100)	<0.0001*	100 (12)	9.07 ± 0.26	0.95	12/12 (100)	4.98 ± 1.03	0.16
5	1	9.49	MFA	37/56 (66)	<0.0001*	100 (12)	9.11 ± 0.13	0.95	12/12 (100)	5.52 ± 0.66	0.16
8	1	9.21	DSFA	30/30 (100)	<0.0001*	9/9 (100)	9.16 ± 0.09	0.02*	9/9(100)	4.67 ± 0.94	0.02*
8	1	9.21	MFA	27/56 (48.2)	<0.0001*	12/12 (100)	8.45 ± 0.54	0.02*	12/12(100)	5.24 ± 0.28	0.02*
9	1	5.65	DSFA	27/30 (90)	0.03*	12/12 (100)	3.62 ± 1.93	0.88	0/12	0	ND
9	1	5.65	MFA	38/56 (67.8)	0.03*	8/12 (66.7)	4.57 ± 3.10	0.88	0/12	0	ND
10	1	4.76	DSFA	30/30 (100)	0.007*	2/12 (16.7)	3.52	ND	0/12	0	ND
10	1	4.76	MFA	45/56 (80.4)	0.007*	2/12 (16.7)	3.08	ND	0/12	0	ND
11	1	7.51	DSFA	29/30 (96.7)	0.25	12/12 (100)	6.22 ± 2.96	0.52	6/12 (50)	3.95 ± 0.7	0.09
11	1	7.51	MFA	49/56 (87.5)	0.25	12/12 (100)	6.38 ± 3.3	0.52	5/12 (42)	4.95 ± 0.76	0.09
12	1	9.18	DSFA	28/30 (93.3)	1	12/12 (100)	9.8 ± 0.48	0.52	12/12 (100)	5.18 ± 0.49	0.003
12	1	9.18	MFA	51/56 (91.1)	1	12/12 (100)	9.66 ± 0.31	0.52	8/12 (67)	4.74 ± 1.66	0.003
13	1	8.44	DSFA	17/17 (100)	0.57	12/12 (100)	9.69 ± 0.37	0.06	11/12 (92)	3.52 ± 1.19	0.82
13	1	8.44	MFA	43/47 (91.5)	0.57	12/12 (100)	9.73 ± 1.51	0.06	12/12 (100)	4.89 ± -.66	0.82
14	1	5.71	DSFA	17/17 (100)	0.001*	7/12 (58.3)	3.25 ± 1.12	1	0/12	0	ND
14	1	5.71	MFA	27/46 (58.7)	0.001*	8/12 (66.7)	2.83 ± 0.2	1	0/12	0	ND
15	1	5.64	DSFA	16/17 (94.1)	0.66	11/11 (100)	5.21 ± 3.00	0.14	3/12 (27)	4.19 ± 0.57	ND
15	1	5.64	MFA	37/42 (88.1)	0.66	9/12 (75)	3.87 ± 2.01	0.14	1/11 (9)	3.95	ND
16	1	8.85	DSFA	17/17 (100)	1	12/12 (100)	9.50 ± 0.11	0.02*	12/12 (100)	4.64 ± 0.49	0.8
16	1	8.85	MFA	46/46 (100)	1	12/12 (100)	9.66 ± 0.17	0.02*	12/12 (100)	4.34 ± 0.67	0.8
17	1	4.75	DSFA	17/17 (100)	0.02*	8/12 (66.7)	2.62 ± 0.01	0.16	0/12	0	ND
17	1	4.75	MFA	40/56 (71.4)	0.02*	7/12 (58.3)	3.48 ± 0.82	0.16	0/12	0	ND
18	1	7.55	DSFA	17/17 (100)	0.03*	12/12 (100)	7.6 ± 3.68	0.06	1/12 (8)	4.95	ND
18	1	7.55	MFA	42/56 (75)	0.03*	12/12 (100)	5.44 ± 3.29	0.06	6/12 (50)	3.73 ± 0.30	ND
19	1	7.31	DSFA	17/17 (100)	0.02*	10/12 (83.3)	5.9 ± 3.26	0.45	5/12 (45)	4.69 ± 0.74	0.76
19	1	7.31	MFA	39/54 (72.2)	0.02*	10/11 (90.9)	6.89 ± 3.17	0.45	3/12 (25)	4.52 ± 1.00	0.76
20	1	8.2	DSFA	17/17 (100)	0.32	12/12 (100)	9.66 ± 0.24	0.15	12/12 (100)	4.25 ± 0.49	0.54
20	1	8.2	MFA	50/56 (89.3)	0.32	12/12 (100)	9.51 ± 0.18	0.15	12/12 (100)	4.36 ± 0.52	0.54
21	1	8.79	DSFA	17/17 (100)	0.19	12/12 (100)	8.86 ± 0.72	0.4529	12/12 (100)	4.22 ± 0.41	0.63
21	1	8.79	MFA	49/56 (87.5)	0.19	12/12 (100)	9.12 ± 0.22	0.4529	12/12 (100)	4.26 ± 0.59	0.63
22	1	8.84	DSFA	17/17 (100)	0.02*	12/12 (100)	9.17 ± 0.12	0.05	12/12 (100)	5.02 ± 0.34	0.033
22	1	8.84	MFA	40/56 (71.4)	0.02*	12/12 (100)	9.37 ± 0.27	0.05	12/12 (100)	4.52 ± 0.65	0.033
23	1	9.39	DSFA	17/17 (100)	0.58	12/12 (100)	9.05 ± 0.21	0.1489	12/12 (100)	3.58 ± 0.71	0.42
23	1	9.39	MFA	51/56 (91.1)	0.58	12/12 (100)	9.14 ± 0.41	0.1489	12/12 (100)	3.9 ± 0.44	0.42
24	1	6.31	DSFA	17/17 (100)	0.17	12/12 (100)	5.58 ± 2.83	0.8399	3/12 (17)	3.84 ± 1.33	ND
24	1	6.31	MFA	42/56 (75)	0.17	12/12 (100)	6.1 ± 2.85	0.8399	6/12 (50)	4.40 ± 0.39	ND
25	1	8.12	DSFA	17/17 (100)	1	12/12 (100)	9.26 ± 0.46	0.5637	12/12 (100)	4.75 ± 0.58	0.29
25	1	8.12	MFA	55/58 (94.8)	1	12/12 (100)	9.34 ± 0.26	0.5637	12/12 (100)	4.48 ± 0.46	0.29
26	1	8.03	DSFA	17/17 (100)	1	12/12 (100)	9.03 ± 0.34	0.3865	12/12 (100)	4.99 ± 0.50	0.4
26	1	8.03	MFA	55/56 (98.2)	1	12/12 (100)	8.16 ± 2.26	0.3865	10/12 (83)	5.15 ± 0.41	0.4
4	2	5.6	DSFA	29/30 (96.7)	1	7/12 (58.3)	3.46 ± 2.34	1	1/12(8.3)	4.52	ND
4	2	5.6	MFA	55/56 (98)	1	7/12 (58.3)	3.52 ± 2.12	1	1/12(8.3)	2.52	ND
7	2	3.73	DSFA	29/29 (100)	0.09	11/12 (91.7)	3.04 ± 0.52	0.21	0/12	0	ND
7	2	3.73	MFA	51/58 (87.9)	0.09	9/12 (75)	3.42 ± 0.65	0.21	0/12	0	ND
1	3	3.69	DSFA	30/30 (100)	0.15	1/12 (8.3)	2.47	ND	0/12	0	ND
1	3	3.69	MFA	47/56 (83.9)	0.15	2/12 (16.7)	3.11	ND	0/12	0	ND
2	3	3.94	DSFA	30/30 (100)	<0.0001*	3/12 (25)	1.68 ± 0.38	ND	0/12	0	ND
2	3	3.94	MFA	39/59 (69.6)	<0.0001*	2/12 (16.7)	1.41	ND	0/12	0	ND
3	3	8.69	DSFA	30/30 (100)	0.0001*	12/12 (100)	9.23 ± 0.18	0.15	12/12 (100)	5.22 ± 1.26	0.16
3	3	8.69	MFA	40/60 (66.7)	0.0001*	12/12 (100)	9.34 ± 0.18	0.15	12/12 (100)	4.54 ± 1.14	0.16

^aFisher's Exact Test (P- value < 0.05 are highlighted*), ^bPairwise comparison of midgut and salivary glands infection rate from each patient showed no significant differences by Fisher's Exact Test, ND Not done

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