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Table 2 Relative sterol content of a recently isolated virulent (vir) strain compared to an avirulent (L5) strain of Leishmania infantum

From: Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form

Parasite-growth phase L5-LOG (n = 2) Vir-LOG (n = 3) L5-STAT (n = 5) Vir-STAT (n = 11) L5-STAT (n = 3) Vir-STAT (n = 3)
Growth Medium HOMEM SFM
Sterol:       
Cholesterol 16.68 (3.70) 14.60 (0.38) 33.69 (5.25) 27.77 (7.94) 1.14 (0.13) 3.69 (4.28)
Zymosterol 0.00 (0.00) 0.00 (0.00) 0.27 (0.38) 0.71 (2.17) 0.72 (0.63) 2.52 (2.25)
Ergosterol -I 28.68 (6.48) 30.92 (3.73) 18.99 (3.85) 19.09 (7.59) 22.96 (3.09) 34.70* (5.98)
Ergosta-7,22-dien-3β-ol - I 5.62 (6.48) 4.76 (3.74) 2.29 (1.58) 5.50 (4.64) 2.16 (1.94) 1.64 (1.33)
Ergostatetraenol 3.27 (4.62) 3.38 (3.57) 1.92 (1.72) 0.72 (1.25) 1.97 (1.80) 3.39 (2.39)
Ergosterol -II 39.93 (2.62) 39.13 (1.37) 39.28 (5.81) 21.74** (11.33) 50.17 (5.33) 31.33* (8.09)
Ergosta-7,22-dien-3β-ol- II 1.71 (0.04) 6.58* (1.20) 0.84 (0.66) 20.66*** (9.43) 12.69 (9.50) 12.56 (7.28)
Lanosterol 0.00 (0.00) 0.00 (0.00) 0.16 (0.36) 0.15 (0.35) 1.14 (1.07) 0.58 (1.00)
Stigmasta-7,24(28)-dien-3β-ol 4.10 (5.80) 0.63 (0.32) 2.50 (3.44) 3.65 (1.88) 4.77 (2.50) 9.60 (5.17)
  1. Data show the average (standard deviation) percentages of each sterol relative to the total amount of sterols in each cell type, set at 100 %. Sterols were identified and quantified by gas chromatograph-mass spectrometry. L. infantum virulent (vir) or avirulent L5 promastigotes were cultured in either HOMEM or serum free medium (SFM) and harvested for sterol analyses in logarithmic (LOG) or stationary (STAT) growth phases
  2. *P < 0.05; **P < 0.01; ***P < 0.001