Fig. 3

mRNA expression levels of cytokines from L. amazonensis-infected footpads measured by qPCR. Wild type (WT) and gp91^phox−/− mice were infected with 1 × 10⁶ metacyclic promastigote forms of L. amazonensis in the right hind footpad and followed for 16 weeks. a, b, c, d and e represent the mRNA expression of IFN-γ, TNF-α, IL-4, IL-10 and IL-1β, respectively, normalized by 18S mRNA expression in each time point. The results were expressed by mean ± SD, n = 5, *P < 0.05 between WT and gp91^phox−/− mice