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Fig. 7 | Parasites & Vectors

Fig. 7

From: Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection

Fig. 7

Myeloperoxidase (MPO) activity and Ly6G+ cells at the infection site in wild type (WT) and gp91phox−/− mice. Mice were infected with 1 × 106 metacyclic promastigote forms of L. amazonensis (PH8) in the right hind footpad, or injected with PBS. a After 6 h or 72 h of infection, footpads were removed and used to assay MPO activity to estimate neutrophil numbers in the acute phase of infection. b Flow cytometry of Ly6G+ cells in footpads 4, 8, 12 and 16 weeks post-infection, performed as described in the Methods section. Data are shown as mean ± SD from one representative experiment of two, n = 5 for each experiment, *P < 0.05, **P < 0.01 between WT and gp91phox−/− mice. c Representative dot plots of Ly6G+ cells recovered from infected footpads after 4, 8, 12 and 16 weeks of infection. The dot plots represent percentage of Ly6G+ cells gated on side scatter versus forward scatter cell properties

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