Fig. 7

Myeloperoxidase (MPO) activity and Ly6G⁺ cells at the infection site in wild type (WT) and gp91^phox−/− mice. Mice were infected with 1 × 10⁶ metacyclic promastigote forms of L. amazonensis (PH8) in the right hind footpad, or injected with PBS. a After 6 h or 72 h of infection, footpads were removed and used to assay MPO activity to estimate neutrophil numbers in the acute phase of infection. b Flow cytometry of Ly6G⁺ cells in footpads 4, 8, 12 and 16 weeks post-infection, performed as described in the Methods section. Data are shown as mean ± SD from one representative experiment of two, n = 5 for each experiment, *P < 0.05, **P < 0.01 between WT and gp91^phox−/− mice. c Representative dot plots of Ly6G⁺ cells recovered from infected footpads after 4, 8, 12 and 16 weeks of infection. The dot plots represent percentage of Ly6G⁺ cells gated on side scatter versus forward scatter cell properties