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Table 4 Comparative description of eggs of An. culicifacies species A, D and E showing morphological differences

From: Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

  An. culicifacies ‘A’ An. culicifacies ‘D’ An. culicifacies ‘E’
Colour Black Black Black
Shape Boat-shaped in lateral, ventral and dorsal views Broadly boat-shaped in both ventral and dorsal views Broadly boat-shaped in both ventral and dorsal views
Ventral and dorsal surface Ventral surface concave, dorsal surface curved Ventral surface concave, dorsal surface strongly curved Ventral surface slightly concave, dorsal surface curved
Anterior and posterior ends Anterior and posterior ends blunt, sometimes pointed Anterior end blunt, posterior end slightly pointed and sometimes blunt Anterior end blunt, posterior end slightly pointed, sometimes blunt
Anterior lobed ventral tubercles Usually oval Usually oval or oblong, but occasionally round Usually oval or oblong
Posterior lobed tubercles Similar in structure to anterior lobed tubercles Similar in structure to anterior lobed tubercles Similar in structure to anterior lobed tubercles but sometimes found to be round
Number of antero-ventral tubercles 3–7 2–3 3–4
Number of postero-ventral tubercles 1–2 No. less from No. of antero-ventral tubercles 2–3 (Similar in No. to No. of antero-ventral tubercles) Only 1 No. less from No. of antero-ventral tubercles
Micropyle Outline of micropylar collar irregular in shape and well developed Micropylar collar irregular in outline, with smooth surface Outline of micropylar collar irregular in shape, with slight striations
Number of sectors of micropylar disc Bigger micropyle with 6–7 sectors Only 6 sectors Only 6 sectors
Floats Short and closer to ventral than dorsal surface Relatively short and narrow in dorso-ventral plane Relatively long and wide in dorso-ventral plane
Deck Continuous, slightly narrows at middle of float, anterior part of deck usually as wide as posterior part Continuous, anterior part of deck usually wider than posterior part Continuous and anterior part of deck usually wider than middle part