Protection against Schistosoma mansoni infection using a Fasciola hepatica -derived fatty acid binding protein from different delivery systems

Parasites & Vectors

Table 2 Cytokine production (TNF-α, IL-6, IL-1α IFNγ, IL-2, IL-4, IL-10, IL-17) in supernatants of splenocyte cultures in untreated BALB/c mice, treated with AA0029+Qs and immunised with AA0029+Qs+rFh15 and AA0029+Qs+rFh15b 2 weeks after immunisation schedule. Data are presented as the mean ± standard error of the mean. Kruskal-Wallis χ ², degrees of freedom (df) and P-values, and P-values in case of significant differences in pairwise comparisons

Cytokine	Untreated	AA0029+Qs	AA0029+Qs+rFh15	AA0029+Qs+rFh15b	Kruskal-Wallis
	(pg/ml)	(pg/ml)	(pg/ml)	(pg/ml)	χ ²	df	P
TNFα	313 ± 98	214 ± 20	937 ± 130	1,074 ± 89	16.22	3	0.001
TNFα	313 ± 98	214 ± 20	P = 0.001^a	P = 0.001^a	16.22	3	0.001
IL-6	964 ± 118	1,318 ± 137	2,755 ± 226	1,613 ± 137	17.11	3	0.001
			P = 0.001^a	P = 0.001^a
			P = 0.001^a	P < 0.001^b
IL-1α	527 ± 65	368 ± 32	448 ± 23	581 ± 142
IFNγ	543 ± 35	643 ± 16	735 ± 23	890 ± 79	20.40	3	< 0.001
				P < 0.001^a
				P < 0.001^b
IL-2	592 ± 74	774 ± 84	1,025 ± 47	888 ± 41	10.27	3	0.016
IL-2	592 ± 74	774 ± 84	P < 0.001^a	P = 0.001^a	10.27	3	0.016
IL-4	1,138 ± 101	1,508 ± 82	2,078 ± 145	1,653 ± 18	16.91	3	<0.001
IL-4	1,138 ± 101	1,508 ± 82	P < 0.001^a	P < 0.001^a	16.91	3	<0.001
IL-10	481 ± 46	485 ± 39	424 ± 7	459 ± 21
IL-17	1,724 ± 167	2,048 ± 43	2,053 ± 46	1,988 ± 268

^aSignificant differences in comparison with untreated group; ^bSignificant differences compared to AA0029+Qs+rFh15 vaccinated group