Table 1 Primer sets employed for PCR amplification
From: Tick-borne pathogens of zoonotic and veterinary importance in Nigerian cattle
PCR target | Primer | Sequence (5′– 3′) | Reference |
|---|---|---|---|
Theileria/Babesia spp. 18S rDNA | Forward (RLB-F2) | GACACAGGGAGGTAGTGACAAG | [20] |
Reverse (RLB-R2) | Biotin-CTAAGAATTTCACCTCTGACAGT | ||
Ehrlichia/Anaplasma spp. 16S rDNA | Forward (16S8FE) | GGAATTCAGAGTTGGATC(A/C)TGG(C/T)TCAG | [21] |
Reverse (BGA1B-new) | Biotin-CGGGATCCCGAGTTTGCCGGGACTT(C/T)TTCT | [22] | |
Rickettsia spp. 16S rDNA | Forward (Rick-F1) | GAACGCTATCGGTATGCTTAACACA | [23] |
Reverse (Rick-R2) | Biotin-CATCACTCACTCGGTATTGCTGGA |