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Table 2 Genus- and species-specific probes employed for reverse line blotting

From: Tick-borne pathogens of zoonotic and veterinary importance in Nigerian cattle

 

Tick-borne Microorganism’s Genera/Species

Probe Sequence (from 5′–3′)

Tm* (°C)

Reference

1

Ehrlichia/Anaplasma catch-all

GGGGGAAAGATTTATCGCTA

58

[22]

2

Anaplasma bovis

GTAGCTTGCTATG(A/G)GAACA

56–58

[20]

3

Anaplasma centrale

TCGAACGGACCATACGC

61

[22]

4

Anaplasma marginale

GACCGTATACGCAGCTTG

59

[22]

5

Ehrlichia ruminantium

AGTATCTGTTAGTGGCAG

54

[22]

6

Anaplasma sp. (Omatjenne)

CGGATTTTTATCATAGCTTGC

57

[22]

7

Theileria/Babesia catch-all

TAATGGTTAATAGGA(A/G)C(A/G)GTTG

55–59

[25]

8

Babesia catch-all 1

ATTAGAGTGTTTCAAGCAGAC

57

Nijhof (unpublished)

9

Babesia catch-all 2

ACTAGAGTGTTTCAAACAGGC

60

Nijhof (unpublished)

10

Babesia bigemina

CGTTTTTTCCCTTTTGTTGG

58

[24]

11

Babesia bovis

CAGGTTTCGCCTGTATAATTGAG

61

[24]

12

Theileria annulata

CCTCTGGGGTCTGTGCA

62

[20]

13

Theileria buffeli

GGCTTATTTCGG(A/T)TTGATTTT

56–57

[24]

14

Theileria mutans

CTTGCGTCTCCGAATGTT

59

[24]

15

Theileria parva

GGACGGAGTTCGCTTTG

60

[26]

16

Theileria taurotragi

TCTTGGCACGTGGCTTTT

62

[24]

17

Theileria velifera

CCTATTCTCCTTTACGAGT

54

[24]

18

Rickettsia catch-all

TTTAGAAATAAAAGCTAATACCG

54

[27]

  1. *Tm = melting temperature