Fig. 5

Agarose gel analysis of the PCR products generated by RT-PCR assay and alignment of the amino acid sequence of CHIKV. a. 1014 bp PCR products were reverse-transcribed from CHIKV isolated from Ae. aegypti and Ae. albopictus, M: DNA marker, Lane 1: the uninfected LLC-MK2 cells (negative control), Lane 2: the CHIKV stock-infected LLC-MK2 cells (positive control), Lanes 3–6: the infected LLC-MK2 cells from the F1–F4 Ae. aegypti larvae, Lane 7: the uninfected LLC-MK2 cells from the F5 Ae. aegypti larvae, Lanes 8–12: the infected LLC-MK2 cells from the F1–F5 Ae. albopictus larvae, Lane 13: the uninfected LLC-MK2 cells from the F6 Ae. albopictus larvae, Lanes 14–15: the RNA samples from the F4–F5 Ae. aegypti adults positive for CHIKV by qRT-PCR, Lanes 16–17: the RNA samples from the F5–F6 Ae. albopictus adults positive for CHIKV by qRT-PCR, respectively; b. An alignment of amino acid sequences confirmed the virus transmitted vertically in Ae. aegypti and Ae. albopictus as CHIKV IOL with the A226V mutation in E1 gene as indicated by a vertical column