Fig. 4
From: Transmission mechanisms of an emerging insect-borne rickettsial pathogen
PCR detection of rickettsial 17-kDa antigen gene in Whatman™ FTA cards. a Lane 1, 100 bp DNA marker; Lane 2, blank; Lanes 3–7, single disc punch from five different cards exposed to R. felis-infected cat fleas in the presence of blood; Lanes 8–10, blank; Lane 11, environmental control; Lane 12, positive PCR R. felis genomic DNA; b Lane 1, 100 bp DNA marker; Lanes 2, 7–11, blank; Lanes 3, 4, 5, single disc punch from three different cards exposed to R. felis-infected, surface sterilized cats fleas in the absence of blood; Lane 6, positive PCR R. felis genomic DNA; Lane 12, environmental control