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Table 1 Temporal dynamics of rickettsial transmission between co-feeding cat fleas. Cat fleas were either exposed to an infectious bloodmeal for 1, 3, 6 or 12 h, and then divided into feeding capsules containing naïve cat fleas for 24 h (exposure bioassay), or exposed to an infectious bloodmeal for 24 h, and then divided into feeding capsules containing naïve cat fleas for 1, 3, 6 or 12 h (association bioassay). Acquisition of novel infection by recipient fleas was assessed by qPCR. Rickettsial infection loads were determined by quantifying the copy number of a portion of the R. felis 17-kDa antigen gene per individual flea lysate

From: Transmission mechanisms of an emerging insect-borne rickettsial pathogen

  Donor cat fleas Recipient cat fleas
Exposure (h) Prevalence (%) Mean infection load (± SEM) Prevalence (%) Mean infection load (± SEM)
1 48/90 (53) 4.12E4 (± 1.30E4) 0/90 (0) 0
3 60/90 (67) 3.33E4 (± 7.39E3) 0/90 (0) 0
6 62/90 (69) 4.78E4 (± 9.95E3) 3/90 (3) 2.60E3 (± 2.16E3)
12 68/90 (76) 3.27E6 (± 2.79E6) 6/90 (7) 6.12E3 (± 3.12E3)
Association (h)     
1 67/90 (74) 1.27E7 (± 1.12E7) 0/90 (0) 0
3 58/90 (64) 9.23E3 (± 3.12E3) 0/90 (0) 0
6 55/90 (61) 2.80E4 (± 1.35E4) 0/90 (0) 0
12 57/90 (63) 7.66E4 (± 6.24E4) 0/90 (0) 0