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Table 1 Foxes (Pseudalopex spp.) and ticks analyzed for the presence of Leishmania DNA in  Patagonia, Argentina

From: Detection of Leishmania DNA in wild foxes and associated ticks in Patagonia, Argentina, 2000 km south of its known distribution area

Ref.

Species

Locationa

Date

Blood preservation

PCR resultb

Ticks (Amblyomma tigrinum)c

     

Protocol 1

Protocol 2

Females

PCR Result

Males

PCR Result

ZG1

P. griseus

MNBP

26/11/2010

FTA card

Positive

Positive

5

Positive

8

Negative

ZG2

P. griseus

MNBP

26/11/2010

FTA card

Positive

Positive

1

Positive

2

Negative

ZG3

P. griseus

MNBP

29/11/2010

FTA card

Positive

Negative

10

Negative

17

Negative

ZG4

P. griseus

MNBP

29/11/2010

FTA card

Positive

Negative

5

Positive

6

Positive

ZG5

P. griseus

MNBP

02/12/2010

FTA card

Positive

Negative

2

Positive

2

Positive

ZC1

P. culpaeus

MNBP

20/02/2011

FTA card

Negative

Negative

7

Negative

6

Positive

ZG6

P. griseus

MNBP

11/04/2011

FTA card

Positive

Positive

0

-

1

Positive

ZG7

P. griseus

MNBP

11/04/2011

FTA card

Positive

Negative

1

Negative

1

Positive

ZG8

P. griseus

MNBP

12/04/2011

FTA card

Negative

Negative

0

-

0

-

ZC2

P. culpaeus

MNBP

14/04/2011

-

-

-

1

Positive

0

-

ZG10

P. griseus

MNBP

16/04/2011

FTA card

Positive

Negative

0

-

0

-

ZG11

P. griseus

MNBP

18/04/2011

FTA card

Negative

Negative

0

-

0

-

ZG12

P. griseus

MNBP

02/04/2012

FTA card

Negative

-

0

-

0

-

ZG13

P. griseus

MNBP

02/04/2012

FTA card

Negative

-

1

Positive

0

-

ZG14

P. griseus

MNBP

05/04/2012

FTA card

Negative

-

0

-

0

-

ZG15

P. griseus

MNBP

06/04/2012

FTA card

Negative

-

0

-

0

-

ZG16

P. griseus

MNBP

08/04/2012

FTA card

Positive

-

0

-

0

-

ZG17

P. griseus

MNBP

16/04/2012

FTA card

Negative

-

0

-

0

-

ZG18

P. griseus

MNBP

17/04/2012

FTA card

Negative

Negative

0

-

0

-

ZG19

P. griseus

MNBP

17/04/2012

FTA card

Negative

Negative

0

-

0

-

ZG20

P. griseus

MNBP

19/04/2012

FTA card

Negative

Negative

0

-

0

-

ZG21

P. griseus

MNBP

23/08/2012

FTA card

-

Negative

0

-

0

-

ZG22

P. griseus

MNBP

14/09/2012

FTA card

-

Negative

0

-

0

-

ZG23

P. griseus

MNBP

15/09/2012

FTA card

-

Negative

0

-

0

-

ZG24

P. griseus

MNBP

17/09/2012

FTA card

-

Negative

0

-

0

-

ZG25

P. griseus

MNBP

18/09/2012

FTA card

-

Negative

0

-

0

-

ZG26

P. griseus

ML

28/09/2013

95 % ethanol

-

Positive

0

-

0

-

ZG27

P. griseus

ML

28/09/2013

95 % ethanol

-

Positive

0

-

0

-

ZG28

P. griseus

ML

01/10/2013

95 % ethanol

-

Negative

0

-

0

-

ZG29

P. griseus

ML

01/10/2013

95 % ethanol

-

Positive

0

-

0

-

ZG30

P. griseus

ML

03/10/2013

95 % ethanol

-

Negative

0

-

0

-

ZG31

P. griseus

ML

03/10/2013

95 % ethanol

-

Negative

0

-

0

-

ZG32

P. griseus

ML

03/10/2013

95 % ethanol

-

Negative

0

-

0

-

ZG33

P. griseus

ML

03/10/2013

95 % ethanol

-

Negative

0

-

0

-

  1. aMNBP: Monumento Natural Bosques Petrificados National Park; ML: Monte León National Park
  2. bProtocol 1: perfomed at Facoltá di Medicina Veterinaria, Universitá degli Studi di Torino, Italia; Protocol 2: perfomed at VetGenomics, Barcelona, Spain
  3. cTicks were analyzed in pools. Results refer to the diagnostic by Protocol 1. Presence of Leishmania DNA in tick from ZG2 was also confirmed by Protocol 2
  4. Positive cases are bold