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Table 1 Foxes (Pseudalopex spp.) and ticks analyzed for the presence of Leishmania DNA in  Patagonia, Argentina

From: Detection of Leishmania DNA in wild foxes and associated ticks in Patagonia, Argentina, 2000 km south of its known distribution area

Ref. Species Locationa Date Blood preservation PCR resultb Ticks (Amblyomma tigrinum)c
      Protocol 1 Protocol 2 Females PCR Result Males PCR Result
ZG1 P. griseus MNBP 26/11/2010 FTA card Positive Positive 5 Positive 8 Negative
ZG2 P. griseus MNBP 26/11/2010 FTA card Positive Positive 1 Positive 2 Negative
ZG3 P. griseus MNBP 29/11/2010 FTA card Positive Negative 10 Negative 17 Negative
ZG4 P. griseus MNBP 29/11/2010 FTA card Positive Negative 5 Positive 6 Positive
ZG5 P. griseus MNBP 02/12/2010 FTA card Positive Negative 2 Positive 2 Positive
ZC1 P. culpaeus MNBP 20/02/2011 FTA card Negative Negative 7 Negative 6 Positive
ZG6 P. griseus MNBP 11/04/2011 FTA card Positive Positive 0 - 1 Positive
ZG7 P. griseus MNBP 11/04/2011 FTA card Positive Negative 1 Negative 1 Positive
ZG8 P. griseus MNBP 12/04/2011 FTA card Negative Negative 0 - 0 -
ZC2 P. culpaeus MNBP 14/04/2011 - - - 1 Positive 0 -
ZG10 P. griseus MNBP 16/04/2011 FTA card Positive Negative 0 - 0 -
ZG11 P. griseus MNBP 18/04/2011 FTA card Negative Negative 0 - 0 -
ZG12 P. griseus MNBP 02/04/2012 FTA card Negative - 0 - 0 -
ZG13 P. griseus MNBP 02/04/2012 FTA card Negative - 1 Positive 0 -
ZG14 P. griseus MNBP 05/04/2012 FTA card Negative - 0 - 0 -
ZG15 P. griseus MNBP 06/04/2012 FTA card Negative - 0 - 0 -
ZG16 P. griseus MNBP 08/04/2012 FTA card Positive - 0 - 0 -
ZG17 P. griseus MNBP 16/04/2012 FTA card Negative - 0 - 0 -
ZG18 P. griseus MNBP 17/04/2012 FTA card Negative Negative 0 - 0 -
ZG19 P. griseus MNBP 17/04/2012 FTA card Negative Negative 0 - 0 -
ZG20 P. griseus MNBP 19/04/2012 FTA card Negative Negative 0 - 0 -
ZG21 P. griseus MNBP 23/08/2012 FTA card - Negative 0 - 0 -
ZG22 P. griseus MNBP 14/09/2012 FTA card - Negative 0 - 0 -
ZG23 P. griseus MNBP 15/09/2012 FTA card - Negative 0 - 0 -
ZG24 P. griseus MNBP 17/09/2012 FTA card - Negative 0 - 0 -
ZG25 P. griseus MNBP 18/09/2012 FTA card - Negative 0 - 0 -
ZG26 P. griseus ML 28/09/2013 95 % ethanol - Positive 0 - 0 -
ZG27 P. griseus ML 28/09/2013 95 % ethanol - Positive 0 - 0 -
ZG28 P. griseus ML 01/10/2013 95 % ethanol - Negative 0 - 0 -
ZG29 P. griseus ML 01/10/2013 95 % ethanol - Positive 0 - 0 -
ZG30 P. griseus ML 03/10/2013 95 % ethanol - Negative 0 - 0 -
ZG31 P. griseus ML 03/10/2013 95 % ethanol - Negative 0 - 0 -
ZG32 P. griseus ML 03/10/2013 95 % ethanol - Negative 0 - 0 -
ZG33 P. griseus ML 03/10/2013 95 % ethanol - Negative 0 - 0 -
  1. aMNBP: Monumento Natural Bosques Petrificados National Park; ML: Monte León National Park
  2. bProtocol 1: perfomed at Facoltá di Medicina Veterinaria, Universitá degli Studi di Torino, Italia; Protocol 2: perfomed at VetGenomics, Barcelona, Spain
  3. cTicks were analyzed in pools. Results refer to the diagnostic by Protocol 1. Presence of Leishmania DNA in tick from ZG2 was also confirmed by Protocol 2
  4. Positive cases are bold