Fig. 2
From: Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation
Example of a species-specific FRET-based real-time PCR result. OWCL species were differentiated by melting curve analysis. Single peaks visualized in L. aethiopica (Tm = 55.2 ± 0.5 °C) strains were distinguished from those of L. major (Tm = 57.4 ± 0.2 °C) or L. tropica with the latter showing double peaks (Tm = 66.6 ± 0.1 °C and 48.1 ± 0.5 °C or 55.8 ± 0.6 °C)