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Table 2 Primers and probes for the simultaneous detection and identification of OWCL species

From: Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation

 

Name

Sequence (5’ ➔ 3’)

Function

Primers

cpb F

CGGCARCATCGAGTCGC

S

 

cpb R

GGTCCCGTTCATGTTTCG

AS

Fluorescent-labeled probes

cpb sensor 2

TCGAACGCCTGCAGCATC—FL

AS

 

cpb anchor 2

LC640-GCCCGCCGCCGCAA—PH

AS

  1. S sense sequence, AS antisense sequence
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Parasites & Vectors

ISSN: 1756-3305

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