Table 3 Melting curve temperatures obtained from FRET-based real-time PCR assay
From: Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation
Species-specific | |||
Species | Strain | Peak | Tm (°C) |
Leishmania aethiopica | MHOM/ET/96/WR2315 | Single | 54.5 |
MHOM/SD/99/WR2885 | Single | 54.5 | |
MHOM/PH/2010/WR2970 | Single | 55.3 | |
MHOM/ET/83/130-83 | Single | 55.5 | |
MHOM/ET/90/DISKO | Single | 55.5 | |
MHOM/ET/70/L96 | Single | 55.5 | |
MHOM/ET/81/1091-81 | Single | 55.5 | |
MPRV/ET/71/L111 | Single | 55.5 | |
L. tropica | MHOM/IQ/65/L75 | Double | 55.3 | 66.8 |
MHOM/SU/74/K27 | Double | 47.5 | 66.5 | |
I000/IL/98/LRC-L757 | Double | 55.5 | 66.8 | |
MHOM/AF/06/NRCP2559 | Double | 47.3 | 66.5 | |
MHOM/AF/05/NRCP358 | Double | 48.5 | 66.5 | |
MHOM/SU/66/III | Double | 48.5 | 66.5 | |
MHOM/KE/91/EB135 | Double | 56.5 | 66.5 | |
MHOM/MA/95/LEM3015 | Double | 48.0 | 66.5 | |
MHOM/IR/2000/LEM4036 | Double | 48.5 | 66.5 | |
L. major | MHOM/IL/81/Friedlin | Single | 57.0 |
MHOM/DZ/05/NRCP684 | Single | 57.3 | |
MHOM/BF/06/NRCP2082 | Single | 57.5 | |
MHOM/BF/06/NRCP2204 | Single | 57.3 | |
MHOM/TN/06/NRCP248 | Single | 57.3 | |
MHOM/BZ/05/NRCP2620 | Single | 57.3 | |
MRHO/SU/59/P-STRAIN | Single | 57.3 | |
MHOM/SU/73/29-ASKH | Single | 57.5 | |
MHOM/IL/83/IL24 | Single | 57.5 | |
MTAT/KE/00/T4 | Single | 57.5 | |
MHOM/DZ/89/LIPA228 | Single | 57.5 | |
Non-species specific (causing visceral or mucocutaneous clinical manifestations) | |||
Species | Strain | Peak | Tm (°C) |
L. chagasi | MHOM/BR/74/M2682 | Non-specific single | 44.5 |
L. infantum | MHOM/TN/80/IPT1 | Non-specific single | 44.5 |
MHOM/MA/67/ITMAP263 | Non-specific single | 44.5 | |
L. donovani | MHOM/IN/80/DD8 | Non-specific single | 45.8 |
MHOM/KE/55/LRC-L53 | Non-specific single | 44.5 | |
MHOM/IQ/77/BUMM3 | Non-specific single | 44.5 | |
MHOM/YE/86/LEM934 | Non-specific single | 45.8 | |
MHOM/SD/90/2828 | Non-specific single | 44.5 | |
L. mexicana | MHOM/BZ/82/BEL21 | None | - |
MNYC/BZ/62/M379 | None | - | |
L. panamensis | MHOM/PA/71/LS94 | None | - |
L. guyanensis | MHOM/GF/79/LEM85 | None | - |
L. peruviana | MHOM/PE/84/UN56 | None | - |
MHOM/PE/84/LC39 | None | - | |
L. braziliensis | MHOM/PE/90/AC | Undefined peak | - |
MHOM/CO/90/UA482 | None | - | |
MHOM/BR/75/M2904 | None | - | |
MHOM/BR/75/M2903b | None | - | |
Other protozoa | |||
Species | Strain | Peak | Tm (°C) |
Plasmodium falciparum | 10-4881 | None | - |
10-4830 | None | - | |
Plasmodium malariae | 10-3066 | None | - |
Plasmodium ovale | 10-2848 | None | - |
Plasmodium vivax | 99-551 | None | - |
Trypanosoma cruzi | 10-4342 | None | - |
10-3447 | None | - | |
08-3341 | None | - | |
08-2636 | None | - | |
08-2634 | None | - | |
Trypanosoma brucei | 09-255 | None | - |
08-3460 | None | - | |
00-659 (control) | None | - | |
Toxoplasma gondii | RH strain | None | - |
Entamoeba histolytica | ATCC® 30015 | None | - |
Cryptosporidium parvum | Iowa strain | None | - |
Giardia lamblia | ATCC® 30957 | None | - |