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Table 3 Melting curve temperatures obtained from FRET-based real-time PCR assay

From: Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation

Species-specific
Species Strain Peak Tm (°C)
Leishmania aethiopica MHOM/ET/96/WR2315 Single 54.5
  MHOM/SD/99/WR2885 Single 54.5
  MHOM/PH/2010/WR2970 Single 55.3
  MHOM/ET/83/130-83 Single 55.5
  MHOM/ET/90/DISKO Single 55.5
  MHOM/ET/70/L96 Single 55.5
  MHOM/ET/81/1091-81 Single 55.5
  MPRV/ET/71/L111 Single 55.5
L. tropica MHOM/IQ/65/L75 Double 55.3 | 66.8
  MHOM/SU/74/K27 Double 47.5 | 66.5
  I000/IL/98/LRC-L757 Double 55.5 | 66.8
  MHOM/AF/06/NRCP2559 Double 47.3 | 66.5
  MHOM/AF/05/NRCP358 Double 48.5 | 66.5
  MHOM/SU/66/III Double 48.5 | 66.5
  MHOM/KE/91/EB135 Double 56.5 | 66.5
  MHOM/MA/95/LEM3015 Double 48.0 | 66.5
  MHOM/IR/2000/LEM4036 Double 48.5 | 66.5
L. major MHOM/IL/81/Friedlin Single 57.0
  MHOM/DZ/05/NRCP684 Single 57.3
  MHOM/BF/06/NRCP2082 Single 57.5
  MHOM/BF/06/NRCP2204 Single 57.3
  MHOM/TN/06/NRCP248 Single 57.3
  MHOM/BZ/05/NRCP2620 Single 57.3
  MRHO/SU/59/P-STRAIN Single 57.3
  MHOM/SU/73/29-ASKH Single 57.5
  MHOM/IL/83/IL24 Single 57.5
  MTAT/KE/00/T4 Single 57.5
  MHOM/DZ/89/LIPA228 Single 57.5
Non-species specific (causing visceral or mucocutaneous clinical manifestations)
Species Strain Peak Tm (°C)
L. chagasi MHOM/BR/74/M2682 Non-specific single 44.5
L. infantum MHOM/TN/80/IPT1 Non-specific single 44.5
  MHOM/MA/67/ITMAP263 Non-specific single 44.5
L. donovani MHOM/IN/80/DD8 Non-specific single 45.8
  MHOM/KE/55/LRC-L53 Non-specific single 44.5
  MHOM/IQ/77/BUMM3 Non-specific single 44.5
  MHOM/YE/86/LEM934 Non-specific single 45.8
  MHOM/SD/90/2828 Non-specific single 44.5
L. mexicana MHOM/BZ/82/BEL21 None -
  MNYC/BZ/62/M379 None -
L. panamensis MHOM/PA/71/LS94 None -
L. guyanensis MHOM/GF/79/LEM85 None -
L. peruviana MHOM/PE/84/UN56 None -
  MHOM/PE/84/LC39 None -
L. braziliensis MHOM/PE/90/AC Undefined peak -
  MHOM/CO/90/UA482 None -
  MHOM/BR/75/M2904 None -
  MHOM/BR/75/M2903b None -
Other protozoa
Species Strain Peak Tm (°C)
Plasmodium falciparum 10-4881 None -
  10-4830 None -
Plasmodium malariae 10-3066 None -
Plasmodium ovale 10-2848 None -
Plasmodium vivax 99-551 None -
Trypanosoma cruzi 10-4342 None -
  10-3447 None -
  08-3341 None -
  08-2636 None -
  08-2634 None -
Trypanosoma brucei 09-255 None -
  08-3460 None -
  00-659 (control) None -
Toxoplasma gondii RH strain None -
Entamoeba histolytica ATCC® 30015 None -
Cryptosporidium parvum Iowa strain None -
Giardia lamblia ATCC® 30957 None -