Table 4 Real-time PCR detection and quantification of Leishmania infantum and Anaplasma platys DNA in tissue samples from non-pregnant bitches. Starting Quantity (SQ) mean value of parasite load per PCR reaction
Dogs | Blood | Bone marrow | Skin | Uterus | Ovary | |||||
|---|---|---|---|---|---|---|---|---|---|---|
L. infantum (SQ mean) | A. platys (SQ mean) | L. infantum (SQ mean) | A. platys (SQ mean) | L. infantum (SQ mean) | A. platys (SQ mean) | L. infantum (SQ mean) | A. platys (SQ mean) | L. infantum (SQ mean) | A. platys (SQ mean) | |
809546 | 2.44 × 10-3 | – | 2.19 × 10-2 | – | 1.63 × 10 | – | 2.37 × 10-1 | – | 3.03 × 10-2 | – |
43117 | 2.41 × 10-1 | – | 4.93 | – | 8.99 × 10-3 | – | 7.51 | – | 3.09 × 10 | – |
42853 | –b | – | – | 2.25 × 10-1 | – | 3.29 × 10-2 | – | 3.91 × 10-2 | – | – |
43017 | – | – | – | – | – | – | – | 2.24 × 10-1 | – | 5.83 × 10-2 |
43115 | – | – | 2.01 × 10-1 | 1.35 | 2.24 | – | 2.32 × 10-3 | 2.05 | – | 1.55 |
31585 | – | – | – | – | – | – | – | 1.20 × 10-1 | – | 4.80 × 10-2 |
42641 | – | – | – | 7.80 × 10-2 | – | – | 2.17 × 10-3 | 6.80 × 10-2 | – | 5.95 × 10−2 |
31339 | – | – | 2.50 × 10-3 | 1.90 × 10-1 | – | – | – | 2.81 × 10-1 | – | 5.78 × 10-2 |
830178 | – | – | 1.20 × 10-2 | – | 2.59 × 10-2 | – | – | 1.90 × 10-1 | – | 1.88 |
31956a | – | – | – | – | – | – | – | – | – | – |