Fig. 1

Immunoblot analysis of monoclonal antibody protein recognition profiles. a BBMV proteins were resolved in SDS-PAGE and stained with colloidal Coomassie blue. b Western blot analysis of BBMV probed with mAb A-140 (Lane 1), mAb A-78 (Lane 2), mAb A-10 (Lane 3), immune serum generated against the microvillus extract (IS-BBMV) (Lane 4), and naive serum (Lane 5). c MAbs A-78 and A-10 did not show a significant reduction in the intensity of infection of P. berghei in An. albimanus mosquitoes. d A representative pooled data is shown from replicate experiments, mAb A-140 significantly reduced the intensity of infection of mosquitoes (see Table 1 and Additional file 1 for details). e and f Reactivity of mAb A-140 and IS-BBMV following chemical deglycosylation. Membrane strips were treated with 1 or 20 mM sodium periodate, or untreated (control = C). Molecular masses are indicated in kilodaltons (kDa)