Fig. 2

Immunoprecipitation of An. albimanus midgut proteins using mAb A-140. Representative fractions obtained from the immunoprecipitation of midguts using mAb A-140, analyzed by SDS-PAGE (8 %) and stained with colloidal Coomassie blue. The whole midgut was solubilized with RIPA buffer (Lane 1). The insoluble fraction was solubilized with RIPA + SDS 2 % (Lane 2). The proteins shown are those that bound to Protein G agarose during the pre-clearing step (Lane 3). The proteins shown are those that immunoprecipitated with Protein G agarose + mAb A-140 (Lane 4). Molecular masses are indicated in kDa