Table 1 Primers employed for A. marginale identification and characterization

msp5

major surface protein MSP5

F: 5' GCATAGCCTCCCCCTCTTTC 3'

548

[9]

R: 5' TCCTCGCCTTGCCCCTCAGA 3'

F: 5' TACACGTGCCCTACCGACTTA 3'

345

R: 5' TCCTCGCCTTGCCCCTCAGA 3'

msp1α

major surface protein MSP1a

F: 5'GCATTACAACGCAACGCTTGAG 3'

84–87 bp tandem repeats

[10]

R: 5'GCTTTACGCCGCCGCCTGCGCC 3'

dnaA

Chromosomal replication initiation protein

F: 5' GTTCATAAGCGGGAAGGACA 3'

512

[11]

R: 5' CTTGTCTCGGTCTGGCTAGG 3'

ftsZ

Cell division protein FtsZ

F: 5' CCTGACCACCAATCCGTATC 3'

575

R: 5' CCCGTATGAAGCACCGTATC 3'

groEl

Chaperonin GroEL

F: 5' AGCATAAAGCCCGAGGAACCTT 3'

699

R: 5' GCCGAGCATGTCCTTCCTTCTG 3'

lipA

Lipoyl synthase

F: 5' TGTGGATAGGGACGACCTTC 3'

538

R: 5' AAAGTCATCCTCAGCGTGGT 3'

recA

Recombinase A

F: 5' GGGCGGTAACTGTGCTTTTA 3'

579

R: 5' ACGCCCATGTCGACTATCTC 3'

secY

Preprotein translocase subunit SecY

F: 5' TTCACGCTGCTAGCCCTAAT 3'

501

R: 5' TACGAGGGAAATGCCGTTAC 3'

sucB

Dihydrolipoamide acetyltransferase component

F: 5' GAGATAGCATCTCCGGTTGC 3'

808

R: 5' CTCCCCTGGCCTTTTTACTC 3'